Chemical Industry and Engineering Progress ›› 2020, Vol. 39 ›› Issue (4): 1575-1582.DOI: 10.16085/j.issn.1000-6613.2019-1220

• Resources and environmental engineering • Previous Articles     Next Articles

Cell growth and lipid accumulation of Monoraphidium sp. QLZ-3 in walnut shell extracts with carbon dioxide

Hailiang XING1(),Xunzan DONG1,3,Benyong HAN1,Shuxiang GENG2,Delu NING2,Ting MA2,Xuya YU1()   

  1. 1.Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan,China
    2.Yunnan Academy of Forestry and Grassland, Kunming 650051, Yunnan, China
    3.Yunnan Guiao Agricultural Science and Technology Group, Kunming 650100, Yunnan, China
  • Received:2019-07-29 Online:2020-04-28 Published:2020-04-05
  • Contact: Xuya YU

二氧化碳联合核桃壳提取液促进单针藻Monoraphidium sp. QLZ-3的生长和油脂积累

邢海亮1(),董训赞1,3,韩本勇1,耿树香2,宁德鲁2,马婷2,余旭亚1()   

  1. 1.昆明理工大学生命科学与技术学院,云南 昆明 650500
    2.云南省林业与草原科学院,云南 昆明 650051
    3.云南贵澳农业科技集团,云南 昆明 650100
  • 通讯作者: 余旭亚
  • 作者简介:邢海亮(1995—),男,硕士研究生,研究方向为生物质能源。E-mail: 450442132@qq.com
  • 基金资助:
    国家自然科学基金(21766012);云南省重大专项(2018ZG003)

Abstract:

The effect of CO2 on the cell growth and lipid accumulation of Monoraphidium sp. QLZ-3 in walnut shell extracts (WSE) was studied. Experimental results showed that the biomass and lipid productivities of QLZ-3 were 196.85mg/(L·d) and 97.52mg/(L·d) respectively under the presence of 12% CO2, which were enhanced by 1.33-fold and 1.57-fold compared with the control group, respectively. The exogenous CO2 upregulated the expression level of rbcL that was cultivated in WSE, and thus resulted in an increase in the CO2 fixation. In addition, the 12% CO2 increased the utilization of polyphenol, and increased the activities of the ACCase and ME but decreased those of PEPC of QLZ-3 in WSE. The results indicated that exogenous CO2 could enhance the biomass and lipid productivity and reduce the cost of microalgae cultivation in WSE, which provided a new theoretical basis for the utilization of walnut shell and the industrial production of microalgae.

Key words: waste treatment, carbon dioxide, bioreactors, biodiesel, biomass

摘要:

以单针藻Monoraphidium sp. QLZ-3为对象,研究了CO2对微藻在核桃壳提取液(walnut shell extracts,WSE)中生长及油脂积累的影响。结果显示,在12%的CO2条件下,微藻在WSE中的生物量产率及油脂产率达到196.85mg/(L·d)和97.52mg/(L·d),分别是对照组的1.33倍和1.57倍。WSE培养下,外源CO2上调了微藻中核酮糖1,5-二磷酸羧化酶基因(ribulose 1,5-bisphosphate carboxylase/oxygenase,rbcL)的表达量,从而促进了CO2的固定。此外,12% 的CO2提高了微藻对WSE中多酚的利用,同时上调了乙酰辅酶A羧化酶(acetyl coenzyme A carboxylase,ACCase)和苹果酸酶(malic enzyme,ME)活性,下调了磷酸烯醇式丙酮酸羧化酶(phosphoenolpyruvate carboxylase,PEPC)活性。研究表明,CO2可以提高WSE中微藻的生物量产率和油脂产率,降低培养成本,为核桃壳的资源化利用及微藻的工业化生产提供了新的理论基础。

关键词: 废物处理, 二氧化碳, 生物反应器, 生物柴油, 生物质

CLC Number: 

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