Chemical Industry and Engineering Progress ›› 2018, Vol. 37 ›› Issue (04): 1616-1622.DOI: 10.16085/j.issn.1000-6613.2017-1401

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Effects of organic loading on the secretory characteristics of EPS and particle formation

LU Jia, LIU Yongjun, LIU Zhe, HAO Wei   

  1. School of Environmental & Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, Shaanxi, China
  • Received:2017-07-07 Revised:2017-08-16 Online:2018-04-05 Published:2018-04-05

有机负荷对污泥胞外聚合物分泌特性及颗粒形成的影响

陆佳, 刘永军, 刘喆, 郝伟   

  1. 西安建筑科技大学环境与市政工程学院, 陕西 西安 710055
  • 通讯作者: 刘永军,教授,研究方向为污水处理生物理论与技术。
  • 作者简介:陆佳(1991-),女,硕士研究生。
  • 基金资助:
    国家自然科学基金(51178377)及中国博士后科学基金(2016M602779)项目。

Abstract: Aerobic granular sludge was cultured at 4.8kgCOD/(m3·d) and 1.2kgCOD/(m3·d) to reveal the influence mechanism of different organic loadings on sludge granulation process,by analyzing the secretory characteristics of extracellular polymeric substances(EPS) in aerobic sludge granulation process. Results showed that high organic loading is helpful for promoting the secretion of the EPS,the initial sludge EPS content was 54.82mg/g MLSS,and at the granules formation stage,the EPS content in high organic loading reactor(R1) increased to 220.5mg/g MLSS,and the EPS content in low organic loading reactor(R2) increased to 182.57mg/g MLSS. In addition,high organic loading was also more helpful to increase the content of protein(PN) and polysaccharide(PS),and the change of protein content was more obvious than that of polysaccharides. At the granules formation stage,the PN/PS value in R1 increased from the initial 4.2 to 12.4,and the PN/PS value in R2 increased from the initial 4.2 to 12.19. In combination with three-dimensional fluorescence spectrum and Fourier infrared spectrum analysis,organic loading had no significant effect on the composition of EPS,but it could change the structure of EPS,thus affecting the formation and characteristics of granular sludge.

Key words: aerobic sludge granulation processes, organic loading, extracellular polymeric substance(EPS), three-dimensional fluorescence distributions, protein(PN), mixed liquor suspended solids(MLSS)

摘要: 对比研究了4.8kgCOD/(m3·d)和1.2kgCOD/(m3·d)的有机负荷条件下好氧颗粒污泥的形成过程,并通过解析好氧污泥颗粒化进程中胞外聚合物(extracellular polymeric substances,EPS)的分泌特性,揭示不同有机负荷对污泥颗粒化进程的影响机制。结果表明:高有机负荷更有助于促进细胞分泌EPS,在颗粒形成阶段,高有机负荷反应器(R1)中EPS含量由初始的54.82mg/gMLSS增至220.5mg/gMLSS,低有机负荷反应器(R2)中EPS含量则增至182.57mg/gMLSS;同时,高有机负荷也更有助于蛋白(protein,PN)和多糖(polysaccharide,PS)含量增加,且与多糖相比,蛋白含量增加更为明显,R1中PN/PS值由初始的4.2升高到12.4,而R2中PN/PS值则由初始的4.2升高到12.19,结合三维荧光光谱图和傅里叶红外谱图分析,有机负荷的高低对EPS的组分没有明显影响,但是可以改变EPS的结构,从而影响颗粒污泥的形成及特性。

关键词: 好氧污泥颗粒过程, 有机负荷, 胞外聚合物, 三维荧光分布, 蛋白, 混合液悬浮固体

CLC Number: 

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