Abstract: The multi-function composite doxorubicin-loading liposomes were prepared by the main material of nature soya lecithin,the additional material of cholesterol,the Fe₃O₄ nanoparticles and CdSeTe QDs as well as the drug of DOX.Taking the size and drug entrapment efficiency as index,orthogonal design of five factors four levels was taken to optimize the condition of multi-function composite doxorubicin-loading liposomes.And the five factors were the concentration of liposome,the ratio of drug to lipid,the ultrasound time,the hydration temperature and the quantity of Fe₃O₄.According to optimality condition,the multi-function Composite Doxorubicin-loading Liposomes were prepared by the thin-film dispersion method.And the size,the magnetism and the fluorescence properties of liposomes were detected.The results showed that the size of the multi-function composite doxorubicin-loading liposomes were 200nm and had good fluorescence and perfect magnetic responsibility.

Key words: near-infrared CdSeTe, Fe₃O₄, DOX, orthogonal test, entrapment efficiency

摘要： 以天然大豆卵磷脂为主要材料，胆固醇、近红外量子点CdSeTe和超顺磁性Fe₃O₄粒子为附加材料，DOX为包封的药物，制备了多功能复合载药脂质体。通过正交试验选取影响复合载药脂质体粒径和包封率的5个因素，即磷脂浓度、药脂比、超声时间、水化温度和Fe₃O₄量，每个因素选取4个水平进行优化实验。根据最优条件，采用薄膜分散发制备了多功能复合载药脂质体并对其粒径、磁性和荧光性质进行了测定。结果表明多功能复合载药脂质体粒径大约200nm、近似球形，且具有良好的近红外荧光性能和磁响应性能。

关键词: 近红外CdSeTe, 四氧化三铁, 盐酸阿霉素, 正交试验, 包封率