Extraction and characterization of enzymatic hydrolysis lignin from cellulosic ethanol fermentation residue

doi:10.16085/j.issn.1000-6613.2015.01.015

Chemical Industry and Engineering Progree ›› 2015, Vol. 34 ›› Issue (1): 86-90,159.DOI: 10.16085/j.issn.1000-6613.2015.01.015

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Extraction and characterization of enzymatic hydrolysis lignin from cellulosic ethanol fermentation residue

PAN Qi^1,2, CHEN Jienan¹, ZHANG Xinmin², ZHAN Peng¹, ZHANG Lin¹

1 Bioethanol Research Center of State Forestry Administration, Central South University of Forestry and Technology, Changsha 410004, Hunan, China;
2 School of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, Hunan, China

Received:2014-04-25 Revised:2014-05-25 Online:2015-01-05 Published:2015-01-05

纤维乙醇发酵残渣中酶解木质素的提取与表征

潘奇^1,2, 陈介南¹, 张新民², 詹鹏¹, 张林¹

1 中南林业科技大学国家林业局生物乙醇研究中心, 湖南长沙 410004;
2 中南林业科技大学材料科学与工程学院, 湖南长沙 410004

通讯作者: 陈介南,教授,博士,博士生导师,研究方向为生物能源与生物材料。E-mail chenjnx@gmail.com。
作者简介:潘奇(1989-),男,硕士研究生。
基金资助:
国家林业公益性行业科研专项(201404604),湖南省科技计划重点(2014WK2027)及国家林业科学技术推广项目([2014]55号)。

Abstract

Abstract: The resource of the wastes from cellulosic ethanol production gets more and more attention. Enzymatic hydrolysis lignin (EHL) was extracted and characterized from the residue of bioethanol fermentation using enzymatic poplar fiber. The single factor experiment analysis was conducted to study the effect of alkali concentration, solid-liquid ratio, reaction temperature and time on extraction of EHL. Reaction conditions were optimized by orthogonal analysis. The structure of EHL was characterized using ultraviolet (UV), Fourier Transform-Infrared Spectroscopy (FT-IR). The optimum EHL extraction conditions were NaOH 40g/L, solid-liquid ratio 1:30, reaction temperature 60℃ and reaction time 2.5h. UV and FT-IR spectra showed that the EHL retained intact lignin structure, mainly with syringyl lignin, holding high chemical activities.

Key words: fermentation residue, enzymatic hydrolysis lignin, poplar fiber, structural characterization, cellulosic ethanol

摘要： 近年来,将纤维素乙醇生产过程中所产生的废物进行资源化利用已越来越受重视。本研究利用杨木纤维酶解发酵产纤维素乙醇的残渣进行木质素的提取与表征,采用单因素试验分析碱浓度、料液比、反应温度、反应时间对酶解木质素提取效果的影响,并对反应条件进行正交优化,应用UV、FT-IR光谱仪对分离出的酶解木质素结构进行表征。结果表明:酶解木质素最佳的提取工艺条件为NaOH浓度40g/L、料液比1:30、反应温度60℃、反应时间2.5h。紫外和红外光谱显示酶解木质素保留了完好的木质素结构,以紫丁香基木质素为主,有良好的化学活性。

关键词: 发酵残渣, 酶解木质素, 木质纤维, 结构表征, 纤维乙醇

CLC Number:

TQ31

PAN Qi, CHEN Jienan, ZHANG Xinmin, ZHAN Peng, ZHANG Lin . Extraction and characterization of enzymatic hydrolysis lignin from cellulosic ethanol fermentation residue[J]. Chemical Industry and Engineering Progree, 2015, 34(1): 86-90,159.

潘奇, 陈介南, 张新民, 詹鹏, 张林. 纤维乙醇发酵残渣中酶解木质素的提取与表征[J]. 化工进展, 2015, 34(1): 86-90,159.

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Extraction and characterization of enzymatic hydrolysis lignin from cellulosic ethanol fermentation residue

纤维乙醇发酵残渣中酶解木质素的提取与表征

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