关键词: 合成生物学, 细胞工厂, 类胡萝卜素, 非理性设计, 路径构建, 底盘进化

Abstract: Carotenoids have brilliant colors and strong antioxidant activity, which not only endow these compounds with high commercial value, but also accelerate the construction process for building efficient biosynthetic pathways in heterogeneous chassis. Carotenoids have become an ideal object to develop irrational design strategy. Here we critically review recent advances about carotenoid biosynthesis via irrational design strategies on pathway construction by parts optimization, modular compatibility, and multidimensional regulation etc., as well as chassis evolution by DNA mutation and adaptive evolution. These strategies aimed to break the bottleneck of carotenoid biosynthesis through improving the supply and utilization of precursor GGPP as well as relieving the stress from carotenoid accumulation on microbiological chassis. Based on the demand of employing unconventional chassis and expanding carotenoid kingdom, we also proposed the development of the construction strategies for carotenoid microbiological cell factories towards the automation and intellectualization.

Key words: Synthetic biology, Cell factories, Carotenoids, Irrational design, Pathway construction, Chassis evolution