化工进展 ›› 2020, Vol. 39 ›› Issue (1): 42-48.DOI: 10.16085/j.issn.1000-6613.2019-0604

• 化工过程与装备 • 上一篇    下一篇

智能微流控检测芯片的构建及其Pb2+检测性能

汪伟1,2(),彭减1,林硕1,谢锐1,2,巨晓洁1,2,刘壮1,2,褚良银1,2   

  1. 1. 四川大学化学工程学院,四川 成都 610065
    2. 四川大学高分子材料工程国家重点实验室,四川 成都 610065
  • 收稿日期:2019-04-17 出版日期:2020-01-05 发布日期:2020-01-14
  • 通讯作者: 汪伟
  • 作者简介:汪伟(1984—),男,博士,副教授,博士生导师,研究方向为微流控技术、界面科学、功能材料。E-mail:wangwei512@scu.edu.cn
  • 基金资助:
    国家自然科学基金面上项目(21576167)

Fabrication of smart microfluidic chip and its Pb2+-detection performance

Wei WANG1,2(),Jian PENG1,Shuo LIN1,Rui XIE1,2,Xiaojie JU1,2,Zhuang LIU1,2,Liangyin CHU1,2   

  1. 1. School of Chemical Engineering, Sichuan University, Chengdu 610065, Sichuan, China
    2. State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, Sichuan, China
  • Received:2019-04-17 Online:2020-01-05 Published:2020-01-14
  • Contact: Wei WANG

摘要:

通过将具有Pb2+响应性的聚(N-异丙基丙烯酰胺-共聚-苯并-18冠-6丙烯酰胺)智能微凝胶与H型微通道相结合,构建了一种能便捷、灵敏、可视化检测水溶液中Pb2+浓度的新型智能Pb2+检测微流控芯片。该微流控检测芯片主要由软光刻技术构建,并结合紫外光照聚合在H型微通道中原位构建智能微凝胶。基于该微凝胶的Pb2+响应性体积相变和H型微通道中的流体流动,该微流控检测芯片能将Pb2+浓度信号有效转换为易于检测读取的、可视化的H型微通道中指示液覆盖的指示柱数目的变化信号。通过光学显微镜便捷观察测量指示液覆盖的指示柱数目的变化,实现了对水溶液中痕量Pb2+浓度的超灵敏定量检测。该微流控检测芯片为水环境中的痕量Pb2+浓度的便捷、灵敏、可视化检测提供了新策略。

关键词: 微流体学, 微通道, 凝胶, 体积相变, Pb2+检测信号

Abstract:

This work reported on fabrication of a novel smart microfluidic chip based on integration of Pb2+-responsive poly(N-isopropylacrylamide-co-benzo-18-crown-6-acrylamide) microgel and H-shaped microchannel, for facile, sensitive and visual detection of Pb2+ in water. The microfluidic chip was constructed using soft lithography technique combined with in situ synthesis of the smart microgel via UV-polymerization within the H-shaped microchannel. Based on the Pb2+-responsive volume change of the smart microgel and the flow in the H-shaped microchannel, the microfluidic chip enabled efficient conversion of Pb2+ concentration changes into easily detectable and readable, visual signal of number changes of indicating pillars within the H-shaped microchannel. By using a microscope for observing and measuring the number change of the indicating pillars, sensitive and quantitative detection of Pb2+ concentration in water was achieved. The proposed microfluidic chip provided a new strategy for facile, sensitive and visual detection trace Pb2+ in water.

Key words: microfluidics, microchannel, gel, volume phase transition, Pb2+ detection

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