智能微流控检测芯片的构建及其Pb2+检测性能

doi:10.16085/j.issn.1000-6613.2019-0604

化工进展 ›› 2020, Vol. 39 ›› Issue (1): 42-48.DOI: 10.16085/j.issn.1000-6613.2019-0604

智能微流控检测芯片的构建及其Pb²⁺检测性能

汪伟^1,²(),彭减¹,林硕¹,谢锐^1,²,巨晓洁^1,²,刘壮^1,²,褚良银^1,²

1. 四川大学化学工程学院，四川成都 610065
2. 四川大学高分子材料工程国家重点实验室，四川成都 610065

收稿日期:2019-04-17 出版日期:2020-01-05 发布日期:2020-01-14
通讯作者: 汪伟
作者简介:汪伟（1984—），男，博士，副教授，博士生导师，研究方向为微流控技术、界面科学、功能材料。E-mail：wangwei512@scu.edu.cn。
基金资助:
国家自然科学基金面上项目(21576167)

Fabrication of smart microfluidic chip and its Pb²⁺-detection performance

Wei WANG^1,²(),Jian PENG¹,Shuo LIN¹,Rui XIE^1,²,Xiaojie JU^1,²,Zhuang LIU^1,²,Liangyin CHU^1,²

1. School of Chemical Engineering, Sichuan University, Chengdu 610065, Sichuan, China
2. State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, Sichuan, China

Received:2019-04-17 Online:2020-01-05 Published:2020-01-14
Contact: Wei WANG

摘要/Abstract

摘要：

通过将具有Pb²⁺响应性的聚（N-异丙基丙烯酰胺-共聚-苯并-18冠-6丙烯酰胺）智能微凝胶与H型微通道相结合，构建了一种能便捷、灵敏、可视化检测水溶液中Pb²⁺浓度的新型智能Pb²⁺检测微流控芯片。该微流控检测芯片主要由软光刻技术构建，并结合紫外光照聚合在H型微通道中原位构建智能微凝胶。基于该微凝胶的Pb²⁺响应性体积相变和H型微通道中的流体流动，该微流控检测芯片能将Pb²⁺浓度信号有效转换为易于检测读取的、可视化的H型微通道中指示液覆盖的指示柱数目的变化信号。通过光学显微镜便捷观察测量指示液覆盖的指示柱数目的变化，实现了对水溶液中痕量Pb²⁺浓度的超灵敏定量检测。该微流控检测芯片为水环境中的痕量Pb²⁺浓度的便捷、灵敏、可视化检测提供了新策略。

关键词: 微流体学, 微通道, 凝胶, 体积相变, Pb²⁺检测信号

Abstract:

This work reported on fabrication of a novel smart microfluidic chip based on integration of Pb²⁺-responsive poly(N-isopropylacrylamide-co-benzo-18-crown-6-acrylamide) microgel and H-shaped microchannel, for facile, sensitive and visual detection of Pb²⁺ in water. The microfluidic chip was constructed using soft lithography technique combined with in situ synthesis of the smart microgel via UV-polymerization within the H-shaped microchannel. Based on the Pb²⁺-responsive volume change of the smart microgel and the flow in the H-shaped microchannel, the microfluidic chip enabled efficient conversion of Pb²⁺ concentration changes into easily detectable and readable, visual signal of number changes of indicating pillars within the H-shaped microchannel. By using a microscope for observing and measuring the number change of the indicating pillars, sensitive and quantitative detection of Pb²⁺ concentration in water was achieved. The proposed microfluidic chip provided a new strategy for facile, sensitive and visual detection trace Pb²⁺ in water.

Key words: microfluidics, microchannel, gel, volume phase transition, Pb²⁺ detection

中图分类号:

O648.17

汪伟,彭减,林硕,谢锐,巨晓洁,刘壮,褚良银. 智能微流控检测芯片的构建及其Pb²⁺检测性能[J]. 化工进展, 2020, 39(1): 42-48.

Wei WANG,Jian PENG,Shuo LIN,Rui XIE,Xiaojie JU,Zhuang LIU,Liangyin CHU. Fabrication of smart microfluidic chip and its Pb²⁺-detection performance[J]. Chemical Industry and Engineering Progress, 2020, 39(1): 42-48.

图/表 8

图1 智能Pb2+检测微流控芯片的检测原理

图2 智能Pb2+检测微流控芯片微通道中的凝胶固定柱、紧缩段和指示柱阵列的扫描电镜图

图3 智能Pb2+检测微流控芯片内部微通道结构的光学显微镜图及具有3个独立H型微通道的微流控芯片数码照片

图4 不同温度下指示液覆盖指示柱情况的光学显微镜图

图5 不同温度下指示液覆盖的指示柱数目的变化（ΔN）情况

图6 不同Pb2+浓度下指示液覆盖的指示柱情况的光学显微镜图

图7 指示液覆盖的指示柱数目变化与Pb2+浓度之间的关系

图8 干扰离子对指示液覆盖的指示柱数目变化的影响

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智能微流控检测芯片的构建及其Pb²⁺检测性能

Fabrication of smart microfluidic chip and its Pb²⁺-detection performance

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