Abstract: To develop a practical process for (R)-2-octanol production from racemic 2-octanol，a two step microbial reaction was investigated. The microbial reaction used the eantioselective oxidation of (S)-2-octanol by Candida cylindracea ATCC 14830 and the asymmetric reduction of oxidated 2-octanone by Oenococcus oeni CECT 4730. O. oeni CECT 4730 was a strain reported which can reduce 2-octanone to (R)-2-octanol，C. cylindracea ATCC 14830 was screened as an candidate biocatalyst for preparation of (R)-2-octanol and 2-octanone with the optical purity of 98%e.e. and the yield of 46%. After redox-coupling，the yield and optical purity were 92% and 98% respectively. The substrate concentration was 40g/L.