农村有机生活垃圾干发酵氨胁迫下中试工艺

doi:10.16085/j.issn.1000-6613.2022-1584

化工进展 ›› 2023, Vol. 42 ›› Issue (7): 3847-3854.DOI: 10.16085/j.issn.1000-6613.2022-1584

农村有机生活垃圾干发酵氨胁迫下中试工艺

刘洋¹^,²(), 叶小梅¹^,³, 苗晓⁴, 王成成¹^,², 贾昭炎¹^,², 曹春晖¹^,², 奚永兰¹^,²^,³()

^1.江苏省农业科学院，江苏南京 210014
^2.江苏大学农业工程学院，江苏镇江 212013
^3.农业农村部种养结合重点实验室，江苏南京 210014
^4.徐州市环能生态技术有限公司，江苏徐州 221000

收稿日期:2022-08-26 修回日期:2022-09-29 出版日期:2023-07-15 发布日期:2023-08-14
通讯作者: 奚永兰
作者简介:刘洋（1997—），男，硕士研究生，研究方向为废弃物资源化利用。E-mail：2221916022@stmail.ujs.edu.cn。
基金资助:
国家重点研发计划(2018YFD1100600);江苏省自然科学基金(BK20201242);江苏省农业自主创新项目(CX(21)2015)

Pilot-scale process research on dry digestion of rural organic household waste under ammonia stress

LIU Yang¹^,²(), YE Xiaomei¹^,³, MIAO Xiao⁴, WANG Chengcheng¹^,², JIA Zhaoyan¹^,², CAO Chunhui¹^,², XI Yonglan¹^,²^,³()

^1.Jiangsu Academy of Agriculture Sciences, Nanjing 210014, Jiangsu, China
^2.School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China
^3.Key Laboratory of Crop and Livestock Integration, Ministry of Agriculture and Rural Affairs, Nanjing 210014, Jiangsu, China
^4.Xuzhou Environmental Energy & Ecology Technology Co. , Ltd. , Xuzhou 221000, Jiangsu, China

Received:2022-08-26 Revised:2022-09-29 Online:2023-07-15 Published:2023-08-14
Contact: XI Yonglan

摘要/Abstract

摘要：

我国农村生活垃圾年产量达到2.94亿吨，占比约40%的有机部分需妥善处理。处理效率较高的干式厌氧发酵由于高有机负荷，容易出现酸化和氨氮抑制问题，在中试及以上规模的发酵中更加明显。农村有机生活垃圾与牛粪进行1m³规模的干式厌氧共发酵，卧式厌氧发酵罐内总固体含量为19.94%，对其沼气产量、甲烷含量、甲烷产量、挥发性脂肪酸浓度、氨氮浓度和微生物群落结构进行分析。结果表明发酵过程中未出现酸化和氨氮抑制，单位挥发性固体含量沼气产量达到1.253m³/kg，甲烷含量稳定在70%，甲烷产量达到了0.815m³/kg，远高于已有报道。微生物群落结构分析表明此次干式厌氧发酵中试系统的微生物生长环境良好，Methanosarcina相对丰度发酵全程大于80%，Firmicutes全程高于77%，分别为古菌和细菌的绝对优势菌群，发酵系统具有良好的微生物环境。

关键词: 农村有机生活垃圾, 干式厌氧发酵, 共发酵, 中试规模, 甲烷

Abstract:

The annual output of rural household waste in China reaches 294 million tons, accounting for about 40% of the organic fraction that needs to be properly treated. Dry anaerobic digestion with high processing efficiency is prone to acidification and ammonia inhibition due to high organic load, which is more obvious in the fermentation of pilot scale and above. A pilot-scale dry anaerobic co-digestion test was conducted on rural organic household waste to solve the problem of ammonia inhibition in dry anaerobic digestion, improve the efficiency of methane production, and provide a basis for the feasibility of harmless treatment of rural organic household waste and biogas project construction. Dry anaerobic co-digestion of rural organic household waste with cattle manure was carried out on a 1m³ scale with a total solids content of 19.94% in a horizontal anaerobic digestion tank, and the inoculum was domesticated using rural organic household waste. Biogas production, methane content, methane production, volatile fatty acid concentration, ammonia nitrogen concentration and microbial community structure were analyzed during the experiment. The results showed that acidification and ammonia suppression did not occur during fermentation. The hydrolysis and acidification phases were adequately carried out and the methanogenic bacteria were provided with sufficient substrate for methane production. At the end of the trial the methane production per unit of volatile solids reached 1.253m³/kg, with methane content fluctuating around 70% and methane production per unit of volatile solids reaching 0.815m³/kg, much higher than that had been reported. The microbial community structure analysis showed that the dry anaerobic digestion pilot system had a good microbial growth environment, with the relative abundance of Methanosarcina greater than 80% and Firmicutes greater than 77% throughout the digestion, which were the absolute dominant groups of archaea and bacteria respectively, and the digestion system had a well microbial environment.

Key words: rural organic household waste, dry anaerobic digestion, co-digestion, pilot scale, methane

中图分类号:

X712

刘洋, 叶小梅, 苗晓, 王成成, 贾昭炎, 曹春晖, 奚永兰. 农村有机生活垃圾干发酵氨胁迫下中试工艺[J]. 化工进展, 2023, 42(7): 3847-3854.

LIU Yang, YE Xiaomei, MIAO Xiao, WANG Chengcheng, JIA Zhaoyan, CAO Chunhui, XI Yonglan. Pilot-scale process research on dry digestion of rural organic household waste under ammonia stress[J]. Chemical Industry and Engineering Progress, 2023, 42(7): 3847-3854.

图/表 7

参考文献 35

1	TANG Fangfang, YU Zhaosheng, LI Yang, et al. Catalytic co-pyrolysis behaviors, product characteristics and kinetics of rural solid waste and chlorella vulgaris[J]. Bioresource Technology, 2020, 299: 122636.
2	奚永兰, 刘洋, 高娣, 等. 农村生活垃圾厌氧发酵产沼气潜力研究[J]. 农业工程学报, 2020, 36(23): 222-228.
	XI Yonglan, LIU Yang, GAO Di, et al. Potential of biogas produced from anaerobic fermentation of rural household wastes[J]. Transactions of the Chinese Society of Agricultural Engineering, 2020, 36(23): 222-228.
3	袁彧, 刘研萍, 陆文静, 等. 规模化沼气工程消化效率及碳减排核算[J]. 环境工程学报, 2019, 13(1): 204-212.
	YUAN Yu, LIU Yanping, LU Wenjing, et al. Digestion efficiency and carbon emission reduction accounting for large-scale biogas projects[J]. Chinese Journal of Environmental Engineering, 2019, 13(1): 204-212.
4	LI Yebo, STEPHEN Y P, ZHU Jiying. Solid-state anaerobic digestion for methane production from organic waste[J]. Renewable and Sustainable Energy Reviews, 2011, 15(1): 821-826.
5	QIAN Mingyu, ZHANG Yixin, LI Ruihua, et al. Effects of percolate recirculation on dry anaerobic co-digestion of organic fraction of municipal solid waste and corn straw[J]. Energy & Fuels, 2017, 31(11): 12183-12191.
6	MOMAYEZ F, KARIMI K, TAHERZADEH M. Energy recovery from industrial crop wastes by dry anaerobic digestion: A review[J]. Industrial Crops and Products, 2019, 129: 673-687.
7	CHEN Chuang, ZHENG Dan, LIU Gangjin, et al. Continuous dry fermentation of swine manure for biogas production[J]. Waste Management, 2015, 38: 436-442.
8	刘洋, 叶小梅, 王成成, 等. 农村有机生活垃圾与不同原料厌氧共发酵工艺优化[J]. 化工进展, 2022, 41(5): 2770-2777.
	LIU Yang, YE Xiaomei, WANG Chengcheng, et al. Optimization of anaerobic co-digestion process of rural organic household waste with other substrates[J]. Chemical Industry and Engineering Progress, 2022, 41(5): 2770-2777.
9	YAN M, FOTIDIS I, TIAN H, et al. Acclimatization contributes to stable anaerobic digestion of organic fraction of municipal solid waste under extreme ammonia levels: Focusing on microbial community dynamics[J]. Bioresource Technology, 2019, 286: 121376.
10	PENG Xuya, ZHANG Shangyi, LI Lei, et al. Long-term high-solids anaerobic digestion of food waste: Effects of ammonia on process performance and microbial community[J]. Bioresource Technology, 2018, 262: 148-158.
11	SHAPOVALOV Y B, ZHADAN S, BOCHMANN G, et al. Dry Anaerobic digestion of chicken manure: A review[J]. Applied Sciences, 2020, 10(21): 7825.
12	王炯科, 汤晓玉, 王文国. 餐厨垃圾干式厌氧发酵研究进展[J]. 中国沼气, 2021, 39(3): 35-41.
	WANG Jiongke, TANG Xiaoyu, WANG Wenguo. Research progress of dry anaerobic digestion of food waste[J]. China Biogas, 2021, 39(3):35-41.
13	LIU Yang, XIAO Qingbo, JIA Zhaoyan, et al. Relieving ammonia nitrogen inhibition in high concentration anaerobic digestion of rural organic household waste by Prussian blue analogue nanoparticles addition[J]. Bioresource Technology, 2021, 330: 124979.
14	高娣. 沛县村镇有机生活垃圾干式厌氧发酵研究[D]. 南京: 南京农业大学, 2020.
	GAO Di. Study on dry anaerobic fermentation of organic domestic waste in Peixian Villages[D]. Nanjing: Nanjing Agricultural University, 2020.
15	Yura JO, CAYETANO R D, KIM Gi-Beom, et al. The effects of ammonia acclimation on biogas recovery and the microbial population in continuous anaerobic digestion of swine manure[J]. Environmental Research, 2022, 212: 113483.
16	孟伟, 查金, 张思梦, 等. 餐厨垃圾厌氧消化过程氨氮抑制及缓解办法综述[J]. 环境工程, 2019, 37(12): 177-182.
	MENG Wei, ZHA Jin, ZHANG Simeng, et al. A review of ammonia inhibition and its mitigation methods for anaerobic digestion of food waste[J]. Environmental Engineering, 2019, 37(12): 177-182.
17	WANG Xiaojiao, YANG Gaihe, FENG Yongzhong, et al. Optimizing feeding composition and carbon-nitrogen ratios for improved methane yield during anaerobic co-digestion of dairy, chicken manure and wheat straw[J]. Bioresource Technology, 2012, 120: 78-83.
18	ACOSTA N, KANG I DUH, RABAEY K, et al. Cow manure stabilizes anaerobic digestion of cocoa waste[J]. Waste Management, 2021, 126: 508-516.
19	XING Baoshan, CAO Sifan, HAN Yule, et al. Stable and high-rate anaerobic co-digestion of food waste and cow manure: Optimisation of start-up conditions[J]. Bioresource Technology, 2020, 307: 123195.
20	高娣, 奚永兰, 刘洋, 等. 江苏徐州沛县大屯街道有机生活垃圾的理化性质分析[J]. 江苏农业学报, 2020, 36(4): 965-970.
	GAO Di, XI Yonglan, LIU Yang, et al. Analysis on physical and chemical properties of organic domestic waste in Datun Street, Pei County, Xuzhou City, Jiangsu Province[J]. Jiangsu Journal of Agricultural Sciences, 2020, 36(4): 965-970.
21	APHA. Standard methods for the examination of water and wastewater[M]. APHA, 2005.
22	鲍士旦. 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2000.
	BAO Shidan. Soil and agricultural chemistry analysis[M]. Beijing: China Agriculture Press, 2000.
23	XIAO Youqian, DENG L, YANG Hongnan, et al. Alleviation of ammonia inhibition in dry anaerobic digestion of swine manure[J]. Energy, 2022, 253: 124149.
24	WANG Changzhen, ZHANG Yin, WANG Yongzhao, et al. Comparative studies of non‐noble metal modified mesoporous M‐Ni‐CaO‐ZrO₂ (M= Fe, Co, Cu) catalysts for simulated biogas dry reforming[J]. Chinese Journal of Chemistry, 2017, 35(1): 113-120.
25	吕琛, 袁海荣, 王奎升, 等. 果蔬垃圾与餐厨垃圾混合厌氧消化产气性能[J]. 农业工程学报, 2011, 27(S1): 91-95.
	Chen LYU, YUAN Hairong, WANG Kuisheng, et al. Anaerobic digestion performances of fruit and vegetable waste and kitchen waste[J]. Transactions of the Chinese Society of Agricultural Engineering, 2011, 27(S1): 91-95.
26	焦秀瑶, 黄康祎, 王小铭, 等. 有机生活垃圾多组分联合厌氧降解产甲烷性能研究[J]. 中国环境科学, 2019, 39(3): 1078-1086.
	JIAO Xiuyao, HUANG Kangyi, WANG Xiaoming, et al. Impact of combined anaerobic degradation of multi-component organic domestic waste on methane production performances[J]. China Environmental Science, 2019, 39(3): 1078-1086.
27	CHOI Yongjun, Jeongwon RYU, LEE Sang Rak. Influence of carbon type and carbon to nitrogen ratio on the biochemical methane potential, pH, and ammonia nitrogen in anaerobic digestion[J]. Journal of Animal Science and Technology, 2020, 62(1): 74-83.
28	司哺春, 刘凯强, 林新宇, 等. 直接种间电子传递对缓解厌氧消化抑制效应的研究进展[J]. 农业工程学报, 2020, 36(24): 227-235.
	SI Buchun, LIU Kaiqiang, LIN Xinyu, et al. Research progress of the relief of anaerobic digestion inhibitions based on direct interspecies electron transfer[J]. Transactions of the Chinese Society of Agricultural Engineering, 2020, 36(24): 227-235.
29	冯晶, 荆勇, 赵立欣, 等. 生物炭强化有机废弃物厌氧发酵技术研究[J]. 农业工程学报, 2019, 35(12): 256-264.
	FENG Jing, JING Yong, ZHAO Lixin, et al. Research progress on biochar enhanced anaerobic fermentation technology of organic wastes[J]. Transactions of the Chinese Society of Agricultural Engineering, 2019, 35(12): 256-264.
30	李博文, 朱鸿斌, 郭建斌, 等. 鸟粪石沉淀法脱除氨氮对鸡粪厌氧发酵过程的影响[J]. 农业工程学报, 2021, 37(22): 220-225.
	LI Bowen, ZHU Hongbin, GUO Jianbin, et al. Effect of ammonia nitrogen removal by struvite precipitation method on the anaerobic digestion of chicken manure[J]. Transactions of the Chinese Society of Agricultural Engineering, 2021, 37(22): 220-225.
31	于佳动, 赵立欣, 冯晶, 等. 序批式玉米秸秆牛粪混合厌氧干发酵产甲烷工艺优化研究[J]. 农业工程学报, 2018, 34(S1): 86-92.
	YU Jiadong, ZHAO Lixin, FENG Jing, et al. Study on optimal technology of methane production by sequencing batchdry anaerobic digestion with corn straw and cattle manure[J]. Transactions of the Chinese Society of Agricultural Engineering, 2018, 34(S1): 86-92.
32	HU Yuying, WU Jing, LI Huaizhi, et al. Study of an enhanced dry anaerobic digestion of swine manure: Performance and microbial community property[J]. Bioresource Technology, 2019, 282: 353-360.
33	TONG J, FANG P, ZHANG Junya, et al. Microbial community evolution and fate of antibiotic resistance genes during sludge treatment in two full-scale anaerobic digestion plants with thermal hydrolysis pretreatment[J]. Bioresource Technology, 2019, 288: 121575.
34	SHI Xuchuan, GUO Xianglin, ZUO Jiane, et al. A comparative study of thermophilic and mesophilic anaerobic co-digestion of food waste and wheat straw: process stability and microbial community structure shifts[J]. Waste Management, 2018, 75: 261-269.
35	ZHANG Le, LI Fanghua, KUROKI A, et al. Methane yield enhancement of mesophilic and thermophilic anaerobic co-digestion of algal biomass and food waste using algal biochar: Semi-continuous operation and microbial community analysis[J]. Bioresource Technology, 2020, 302: 122892.

项目	农村有机生活垃圾	牛粪
TS/%	19.49±0.12	40.86±1.31
VS（样品挥发性固体重量/样品重量）/%	74.91±0.21	18.41±0.90
有机碳/g·kg^-1	698.71±0.14	245.68±0.23
全氮/g·kg^-1	27.73±0.11	7.91±0.05
碳氮比	25.20	34.17

项目	农村有机生活垃圾	牛粪
TS/%	19.49±0.12	40.86±1.31
VS（样品挥发性固体重量/样品重量）/%	74.91±0.21	18.41±0.90
有机碳/g·kg^-1	698.71±0.14	245.68±0.23
全氮/g·kg^-1	27.73±0.11	7.91±0.05
碳氮比	25.20	34.17

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农村有机生活垃圾干发酵氨胁迫下中试工艺

Pilot-scale process research on dry digestion of rural organic household waste under ammonia stress

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