一株聚-β-羟基丁酸酯高产菌株的筛选及发酵条件的优化

化工进展

一株聚-β-羟基丁酸酯高产菌株的筛选及发酵条件的优化

王利群1，2，王明慧1，汪云1，卿青1，2，周珏1，张月明1，胡蓉1

1常州大学制药与生命科学学院，江苏常州 213164；2常州市药品制造与质量控制工程重点实验室，江苏常州 213164

出版日期:2013-09-05 发布日期:2013-09-05

Screening and fermentation of a high yield strain for poly-β-hydroxybutyrate

WANG Liqun1，2，WANG Minghui1，WANG Yun1，QING Qing1，2，ZHOU Jue1，ZHANG Yueming1，HU Rong1

1School of Pharmaceutical Engineering and Life Sciences，Changzhou University，Changzhou 213164，Jiangsu，China；2Changzhou Key Laboratory of Pharmaceutical Manufacture and Quality Control Engineering，Changzhou 213164，Jiangsu，China

Online:2013-09-05 Published:2013-09-05

摘要/Abstract

摘要： 为了获得利用廉价碳源在细菌胞内快速大量合成聚-β-羟基丁酸酯（poly-β-hydroxybutyrate，PHB）的菌株，通过苏丹黑染色法，从土壤中分离筛选得到一株聚-β-羟基丁酸酯（PHB）高产菌株CCZU-6X，经形态观察、生理生化实验和16S rDNA序列分析，鉴定该菌为红球菌属（Rhodococcus sp.）。该菌株的最佳发酵条件为10 g/L乳糖、2 g/L硫酸铵、起始pH值7.5、装液量30 mL/250 mL、转速160 r/min、温度30 ℃、发酵时间18 h，在此优化条件下PHB的积累量达到细胞干重的79.52%，产量为4.54 g/L。

关键词: 聚-&beta, -羟基丁酸酯；红球菌属；发酵；优化

Abstract: To obtain a strain which can use inexpensive carbon source to synthesize poly-β- hydroxybutyrate(PHB) in a short time，a bacterium CCZU-6X with high production of poly-β-hydroxybutyrate (PHB) was isolated and purified from soil，which was identified as Rhodococcus sp. according to its morphological，physiological properties and 16S rDNA sequence analysis. The optimum fermentation conditions were found as follows：the most suitable carbon source was 10 g/L lactose，and nitrogen source was 2 g/L ammonium sulfate，initial pH 7.5，250 mL flask containing 30 mL medium，30 ℃，shaking at 160 r/min for 18 h. Under the optimum conditions，the production of PHB was 4.54 g/L，as high as 79.52 % of dried cell weight.

Key words: poly-&beta, -hydroxybutyrate；Rhodococcus sp.；fermentation；optimization

王利群1，2，王明慧1，汪云1，卿青1，2，周珏1，张月明1，胡蓉1. 一株聚-β-羟基丁酸酯高产菌株的筛选及发酵条件的优化[J]. 化工进展.

WANG Liqun1，2，WANG Minghui1，WANG Yun1，QING Qing1，2，ZHOU Jue1，ZHANG Yueming1，HU Rong1. Screening and fermentation of a high yield strain for poly-β-hydroxybutyrate[J]. Chemical Industry and Engineering Progree.