Abstract:

Investigation was carried out to elucidate the in?uence of process parameters on the characteristics of chitosan microspheres prepared by reverse phase suspension cross-linked polymerization. The chitosan droplet solidi?ed and hardened by glutaraldehyde crosslinking agent was dispersed in the oil phase composed of liquid paraffin in the presence of Span 80 surfactant. Iminodiacetic acid（IDA）was attached onto chitosan microspheres activated by epichlorohydrin under basic condition. The results indicated that the optimal epoxy density above 0.15 mmol/g gel was achieved at 10%（f）epichlorohydrin as activating accelerator in the solution consisting of 0.6 mol/L NaOH and 40% DMSO. The study on linkaging of IDA demonstrated that the support was synthesized in the solution composed of 0.6 mol/L IDA and 2.0 mol/L NaOH at 60 ℃ for 6 h，whose adsorption of Cu²⁺ was up to 172.787 g/g gel. The moisture content of the matrix was up to 45.60% and the porosity was 69.45% under the optimized conditions. The novel matrix could be used in immobilized metal ion affinity chromatography as the solid phase.