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金属离子对产琥珀酸放线杆菌NJ113厌氧发酵代谢的影响

郑晓宇,李 建,方晓江,陈可泉,奚永兰,姜 岷   

  1. 南京工业大学生物与制药工程学院,材料化学工程国家重点实验室,江苏 南京 210009
  • 出版日期:2011-07-05 发布日期:2011-07-05

Effect of metal ions on fermentation and metabolization of Actinobacillus Succinogenes NJ113

ZHENG Xiaoyu,LI Jian,FANG Xiaojiang,CHEN Kequan,XI Yonglan,JIANG Min   

  1. State Key Laboratory of Materials-Oriented Chemical Engineering,College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 210009,Jiangsu,China
  • Online:2011-07-05 Published:2011-07-05

摘要:

考察了培养基中分别添加Mg2+Mn2+Co2+ 3种金属离子对Actinobacillus succinogenes NJ113菌体生长及产酸的影响,并进行了代谢通量分析。结果表明培养基中分别添加6 mmol/L Mg2+6 mmol/L Mn2+2 mmol/L Co2+后流向HMP途径的通量r17比对照组分别提高了445.38%176.23 %171.67%,使得还原力不足的矛盾得到缓解;流向C4途径的通量r13比对照组分别提高了57.70%15.94%2.91%;最终使得流向丁二酸的通量r16比对照组分别提高了62.69%18.91%5.01%。此外,关键酶活分析结果显示分别添加Mg2+Mn2+以及Co2+后,PEP羧化激酶(Pck)比活力由对照组的339.18 U/mg分别提高到568.732 U/mg728.049 U/mg339.686 U/mg。最终当培养基中分别添加6 mmol/L Mg2+6 mmol/L Mn2+2 mmol/L Co2+后丁二酸产量分别为27.83 g/L26.27 g/L23.54 g/L,比对照的22.79 g/L分别提高22.11%15.27%以及3.4%

Abstract:

The effects of adding Mg2+Mn2+Co2+ on cell growth and succinic acid production was investigated. The metabolic flux of Actinobacillus succinogenes NJ113 was calculated. It was found that the flux of HMP increased by 445.38%176.23 % and 171.67% after adding 6 mmol/L Mg2+6 mmol/L Mn2+ 2 mmol/L Co2+ respectivelythus the reducing power was better balanced. The flux of C4 was 57.70%15.94% and 2.91% higher respectivelywhich led to the improvement of succinic acid flux by 62.69%18.91% and 5.01%. The key enzyme activity analysis showed that the specific activity of PEP carboxykinasePckreached 568.732 U/mg728.049 U/mg and 339.686 U/mg with 6 mmol/L Mg2+6 mmol/L Mn2+2 mmol/L Co2+ addition respectively. As a resultthe concentration of succinc acid was 27.83 g/L26.27 g/Land 23.54 g/Lwhile the concentration of control was only 22.79 g/L.

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