化工进展

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脂肪酶和青霉素酰化酶催化合成手性化合物的研究进展

薛 屏,曹雪荣   

  1. 宁夏大学能源化重点实验室,宁夏 银川750021
  • 出版日期:2011-07-05 发布日期:2011-07-05

Solvent effect on catalytic synthesis of chiral compouds with lipase and penicillin G acylase

XUE Ping,CAO Xuerong   

  1. Key Laboratory of Energy Resources and Chemical Engineering,Ningxia University,Yinchuan 750021,Ningxia,China
  • Online:2011-07-05 Published:2011-07-05

摘要:

生物酶催化合成手性药物和中间体具有高效、节能和环境友好等突出的优势,本文从提高催化反应效率的角度,评述了非水相酶催化反应的溶剂效应——溶剂体系对酶的活性和对映选择性的调控作用;介绍了动力学控制合成β-内酰胺类抗生素的不同溶剂体系,包括有机物-水共溶剂体系有机物-水不共溶剂体系、反胶束体系和双水相体系。提出利用溶剂效应调控动力学合成体系,会有效提高酶催化合成β-内酰胺类抗生素的产率和合成与水解比S/H值,从而实现底物的有效利用。

Abstract:

Synthesis of chiral drugs and intermediates catalyzed by enzyme has high efficiencyenergy saving and environment-friendliness advantages. This paper reviews the solvent effect on the enzymatic reaction in non-aqueous media with lipasesnamelythe activity and enantioselectivity of enzyme adjusted by solvent system to improve the efficiency of catalytic reaction. The kinetically controlled synthesis of β-lactam antibiotics with penicillin G acylase was introduced in different solvent systemsincluding the organic-water miscible solventsorganic-water immiscible solventsreverse micelles and aqueous two-phase system. The yield of kinetically controlled synthesis of β-lactam antibiotics and synthesis/hydrolysis ratioS/Hcould be enhanced by designing an effective solvent system to better utilize the substrates.

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