改性酵母蛋白制备混凝土发泡剂及其处理条件优化

化工进展

改性酵母蛋白制备混凝土发泡剂及其处理条件优化

王程，霍冀川，雷永林，李娴，袁艳红，刘佳奇，高娟

（西南科技大学生物质材料教育部工程研究中心，四川绵阳 621010）

出版日期:2011-03-05 发布日期:2011-03-05

Preparation of concrete foaming agent by yeast protein modification and optimization of processing condition

WANG Cheng，HUO Jichuan，LEI Yonglin，LI Xian，YUAN Yanhong，LIU Jiaqi，GAO Juan

（Engineering Research Center of Biomass Materials，Ministry of Education，Southwest University of Science and Technology，Mianyang 621010，Sichuan，China）

Online:2011-03-05 Published:2011-03-05

摘要/Abstract

摘要：

以啤酒酵母菌体为原料，利用超声波法提取酵母细胞内蛋白质，选择干热法、热碱法和酶法对酵母蛋白进行改性处理，通过比较改性前后酵母蛋白的泡沫性能可知：3种方法均对酵母蛋白泡沫性能有一定的改善作用，其中浓度为1.5% Ca(OH)₂水解后得到发泡母液的泡沫性能最佳，泡沫体积达到1109.1 mL，稳泡时间达10.1 h。采用响应面实验法中的Box-Behnken设计对影响热碱法改性蛋白发泡体积的3个主要因素[反应温度、反应时间、Ca(OH)₂浓度] 进行优化，结果表明：当水解温度77.88 ℃、水解时间2.39 h、Ca(OH)₂浓度1.34%时泡沫体积达最大，最大预测值1135.1 mL，实际测得平均发泡体积为1127.2 mL，与模型理论预测值相比相对误差在0.72%，所以采用响应面实验法优化得到的参数准确可靠，具有实用价值。

Abstract:

The release of intercellular protein from yeast cells was proposed for protein modification. Saccharomyces cerevisiae protein isolate modified to improve its foaming ability was tested. Dry-heating treatment，thermal alkaline denaturalization and enzymatic hydrolysis were used，and the best results were obtained by using thermal alkaline denaturalization method with Ca(OH)₂ concentration of 1.5%，which increased maximum foam volume to 1109.1 mL，and foam stability time to 10.1 h. According to the Box-Behnken center-united experimental design principles，the method of response surface analysis with 3 factors and 3 levels was adopted. The optimum modification conditions were determined by analyzing the three-dimensional surface plot and contour plot and by solving the regression model equation with Design Expert software，which showed that reaction temperature of 77.88 ℃，time of 2.39 h，and Ca(OH)₂concentration of 1.34% were the best conditions. Under the optimal conditions，the forecasted maximum foam volume was 1135.1 mL，and verified value was 1127.3 mL by confirmative test. The relative error was 0.72%，indicating that the regression equation was valid and reliable.

王程，霍冀川，雷永林，李娴，袁艳红，刘佳奇，高娟. 改性酵母蛋白制备混凝土发泡剂及其处理条件优化 [J]. 化工进展.

WANG Cheng，HUO Jichuan，LEI Yonglin，LI Xian，YUAN Yanhong，LIU Jiaqi，GAO Juan. Preparation of concrete foaming agent by yeast protein modification and optimization of processing condition[J]. Chemical Industry and Engineering Progree.