化工进展

• 材料科学与技术 • 上一篇    下一篇

二步种子溶胀法制备氯霉素分子印迹聚合物微球及其识别性能

左华敏,李 雁,李 璐,解新安,熊明洲   

  1. (华南农业大学食品学院,广东 广州 510642)
  • 出版日期:2011-03-05 发布日期:2011-03-05

Preparation of molecularly imprinted polymer microspheres for chloramphenicol by two-step seed swelling and its recognition performance

ZUO Huamin,LI Yan,LI Lu,XIE Xin’an,XIONG Mingzhou   

  1. (College of Food Science,South China Agricultural University,Guangzhou 510642,Guangdong,China)
  • Online:2011-03-05 Published:2011-03-05

摘要:

以聚苯乙烯乳液为种球、氯霉素为模板分子、甲基丙烯酸为功能单体、二甲基丙烯酸乙二醇酯为交联剂,采用二步种子溶胀法制备了单分散氯霉素分子印迹聚合物微球(MIPMs)。通过扫描电镜(SEM),考察了溶胀比搅拌速度水油比交联剂用量分散剂用量等对MIPMs形态粒径大小及粒径分布的影响。并用静态吸附实验考察了微球的吸附识别性能。结果表明,当溶胀比为40,搅拌速度为150 r/min,水油比在(8∶1)~(11∶1),EDMA用量为20 mmolHEC用量为1.2 g时,可以获得一系列粒径均匀分散性好的MIPMs。当功能单体为4 mmol MAA,交联剂为20 mmol EDMA时,制备的MIPMs识别性能最好,以甲砜霉素为竞争底物,分离因子达到5.57。二步种子溶胀法为水相中MIPMs的制备开辟了一条简捷有效的途径。

Abstract:

Monodisperse molecularly imprinted polymer microsphereMIPMsfor chloramphenicol was prepared by two-step seed swelling using linear polystyrene emulsion as seedsmethacrylic acidMAAas functional monomer and ethylene glycol dimethacrylateEDMAas crosslinker. The influences of swelling ratiostirring speedratio of water to oildosage of cross-linker and dispersant on the morphologysize and size distribution of the MIPMs were investigated by scanning electron microscopySEM. Furthermorestatic adsorption was used to evaluate the recognition properties of the MIPMs. When the swelling ratio was 40stirring speed was 150 r/min aroundratio of water to oil was (81)(111)dosage of cross-linker was 20 mmoldosage of dispersant was 1.2 ga series of uniformly sized MIPMs with good dispersion were obtained. When 4 mmol MAA was used as the functional monomerand the dosage of crosslinker was 20 mmolthe prepared MIPMs exhibited the best recognition performanceand the separation factor was up to 5.57 when thiamphenicol was chosen as the competitive molecules. Two-step seed swelling opened a simple and effective approach to preparing MIPMs in water.

京ICP备12046843号-2;京公网安备 11010102001994号
版权所有 © 《化工进展》编辑部
地址:北京市东城区青年湖南街13号 邮编:100011
电子信箱:hgjz@cip.com.cn
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持:support@magtech.com.cn