Abstract:

Immobilization of Lipozyme TL 100 L on resin was realized by adsorption，and its application in sucrose-6-acetate（S-6-a）synthesis was then investigated. The results showed that nonpolar macroporous resin of HZ-802 was the best carrier among 7 kinds of resins by comparing both protein adsorptivity and transesterification capacity. The S-6-a yield was attained more than 80% in the condition of immobilization temperature 30 ℃，adsorption capacity between 50.47～58.1 mg/g resin，and immobilized enzyme relative water content under 5%. Disposing Lipozyme TL 100 L with 2-propanol at the volume ratio of 0.5∶1，the S-6-a yield of the immobilized enzyme reached 95.4%. And the yield still remained 34.2% when the immobilized enzyme was repeated 8 times. The transesterification followed Ping-Pong Bi Bi mechanism by the studies of apparent michaelis constant K_m′ at different substrate concentrations. Activation energy of the immobilized enzyme by adsorption on HZ-802 was reduced 6.47 kJ/mol compared with the commercial Lipozyme TLIM.