含苯聚羟基脂肪酸酯的生物合成及其分子结构表征

化工进展

含苯聚羟基脂肪酸酯的生物合成及其分子结构表征

黄锦标1，2，尚龙安1

1浙江大学宁波理工学院，浙江宁波 315100；2浙江大学化学工程与生物工程学系，浙江杭州 310027

出版日期:2011-10-05 发布日期:2011-10-05

Biosynthesis and molecular structure characterization of polyhydroxyalkanoates containing benzoyl group

HUANG Jinbiao1，2，SHANG Long’an1

1Ningbo Institute of Technology，Zhejiang University，Ningbo 315100，Zhejiang，China；2Department of Chemical and Biochemical Engineering，Zhejiang University，Hangzhou 310027，Zhejiang，China

Online:2011-10-05 Published:2011-10-05

摘要/Abstract

摘要：

聚羟基脂肪酸酯（PHA）具有可生物降解性和生物相容性，是潜在的医学材料。对聚羟基脂肪酸酯进行改性，可提高其应用范围.采用以苯戊酸和蔗糖为混合碳源培养基培养 Pseudomonas putida KT2442，对其细胞内合成的聚羟基脂肪酸酯进行生物改性，并利用核磁共振、红外色谱和气相色谱分析方法表征了改性的PHA分子结构。结果表明合成的聚羟基脂肪酸酯为含有3-羟基苯戊酸单体的中长链聚羟基脂肪酸酯（PHPhA），该单体在PHA链中含量为1.04%，为进一步的研究打下基础。

Abstract:

Polyhydroxyalkanoates（PHA）with the biodegradability and biocompatibility has become one of the potential medical materials. Its application fields can be enlarged by modifying the PHA’s properties. For this purpose，the PHA was synthesized by the culture of Pseudomonas putida KT2442 with phenylvaleric acid and sucrose as mixed carbon source. The molecular structure of this biomodified PHA was characterized by NMR，IR and GC. The analysis result indicates that the biomodificated PHA is medium-length PHA which contains 3-hydroxyphenylvalerate monomer，and this monomer accounts for 1.04% of the PHA chain. This will be useful reference for further PHA study.

黄锦标1，2，尚龙安1 . 含苯聚羟基脂肪酸酯的生物合成及其分子结构表征[J]. 化工进展.

HUANG Jinbiao1，2，SHANG Long’an1. Biosynthesis and molecular structure characterization of polyhydroxyalkanoates containing benzoyl group[J]. Chemical Industry and Engineering Progree.