化工进展

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中性蛋白复合酶法提取粗老茶叶活性成分

张 利1,刘兴勇2,何林芯1,冯喜文2,唐茂田1   

  1. 1四川理工学院化学与制药工程学院;2四川理工学院材料与化学工程学院
  • 出版日期:2010-08-05 发布日期:2010-08-05

Extraction of active constituents from old tea by compound neutral protease

ZHANG Li 1,LIU Xingyong 2,HE Linxin1,FENG Xiwen2,TANG Maotian1   

  1. 1 School of Chemistry and Pharmaceutical Engineering,Sichuan University of Science & Engineering;2 School of Material and Chemical Engineering,Sichuan University of Science &
    Engineering
  • Online:2010-08-05 Published:2010-08-05

摘要: 以茶多酚、茶多糖和咖啡碱的收率为指标,通过单因素实验,对中性蛋白复合酶法提取粗老茶叶中活性成分的工艺进行了考察。实验结果表明,较佳的工艺条件为:纤维素酶、果胶酶、中性蛋白酶的质量比为1∶1∶3,酶用量相对于粗老茶为0.3 mg/g,体系pH 值为6.0,酶解温度50 ℃,酶解时间为1 h。

Abstract: In order to improve the extration of tea polyphenols,tea polysaccharide and caffine from old tea,a single-factor test was conducted with compound neutral protease method. The optimum extraction conditions were found with the ratio of cellulase,pectase and neutral protease as 1∶1∶3 and with the total enzyme dosage 0.3 mg/g at pH 6.0 under 50 ℃ for 1h enzymolysis.

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