Abstract: Biodenitrification is an effective way to deal with the nitrogen pollution of water bodies. In this paper，an aerobic denitrifier H1 was cultured by using (NH4)2SO4 as the nitrogen source and sodium citrate as the carbon source. After 4d of aerobic denitrification，NH4+-N removal rate reached to 76.92% and COD removal rate reached to 84.29%，indicating that H1 was a heterotrophic nitrification-aerobic denitrifier. When NH4+-N and NO3－-N existed as the nitrogen source together，NH4+-N removal rate reached to higher than 80% after 2d，while NO3－-N removal was lagged apparently，indicating H1 took use of NH4+-N as the nitrogen source preferentially. When H1 was used to deal with domestic sewage，the rapid conversion of organic nitrogen to ammonia nitrogen was observed. The total nitrogen，NH4+-N and COD removal rate could all reach to higher than 90%，indicating that H1 possess great application prospect in the field of sewage treatment.