响应面法优化大豆荚壳异黄酮超声-回流萃取工艺

化工进展

响应面法优化大豆荚壳异黄酮超声-回流萃取工艺

王桃云1，2，胡翠英2，王金虎2，杨倩倩2，陈鹏1

1扬州大学生物科学与技术学院；2苏州科技学院化学与生物工程学院

出版日期:2010-03-05 发布日期:2010-03-05

Optimization of ultrasonic and reflux assisted extraction of isoflavones
from soybean hull using response surface methodology

WANG Taoyun1，2，HU Cuiying2，WANG Jinhu2，YANG Qianqian2，CHEN Peng1

1College of Biological Science and Technology，Yangzhou University；2College of
Chemical and Biological Engineering，Suzhou Science and Technology University

Online:2010-03-05 Published:2010-03-05

摘要/Abstract

摘要： 利用超声萃取和回流萃取方法进行单因素实验，选取提取温度、超声时间和乙醇体积分数为影响因子，应用响应面法（RSM）优化大豆荚壳异黄酮的提取条件。结果表明，大豆荚壳异黄酮优化浸提条件为：提取温度83 ℃、乙醇体积分数为84%、超声时间30 min、大豆荚壳颗粒150 μm、液料比25:1、回流萃取时间90 min、超声功率108 W，大豆荚壳总异黄酮的得率为3.5 mg/g。响应面法大豆荚壳总异黄酮的提取条件优化合理可行，为提高大豆荚壳总异黄酮的提取率提供了依据。

Abstract: Ultrasonic and reflux assisted extraction of isoflavones from sorbean hull（ISH）was investigated. For the single-factor experiments，extraction temperature，concentration of ethanol and ultrasonic time were selected as the influencing factors. Combined with the response surface methodology，the extraction conditions for ISH was optimized as：temperature 83 ℃，ethanol concentration 84%，ultrasonic time 30 min，soybean hull size 150 μm，solvent to material 25:1，reflux extraction time 90 min and ultrasonic power 108 W. Under these optimized conditions，the extraction yield of ISH was up to 3.5 mg/g.

王桃云1，2，胡翠英2，王金虎2，杨倩倩2，陈鹏1. 响应面法优化大豆荚壳异黄酮超声-回流萃取工艺 [J]. 化工进展.

WANG Taoyun1，2，HU Cuiying2，WANG Jinhu2，YANG Qianqian2，CHEN Peng1. Optimization of ultrasonic and reflux assisted extraction of isoflavones
from soybean hull using response surface methodology
[J]. Chemical Industry and Engineering Progree.