Abstract: Amino-functional carriers for enzyme immobilization are reviewed，including natural polymers chitin and chitosan as well as synthetic amino-functional silica gel carriers. Enzyme immobilization with methods of glutaraldehyde crosslinking，carrier pre-activation and enzyme pre-activation are summarized. Development trends for enzyme immobilization using amino-functional carriers are also discussed. To afford more moderate reaction conditions，larger enzyme immobilization amount and higher enzyme activity recovery and stability，it is necessary to further investigate new amino-functional carriers for enzyme immobilization. As for specific carriers and enzymes，immobilized enzyme performance can be further improved through optimized combinations of ligand，stabilizer addition as well as modification before and after immobilization and post-treatment.