Abstract: To enhance efficiency of asymmetric synthesis of (R)-2-octanonol with Oenococcus oeni CECT 4730，we applied in situ absorption technique to compose an aqueous/resin system for the reaction. The most suitable resin AB-8 was selected from 6 types of absorption resins with respect to the effect on the bioreduction. After evaluated effect of the load of resin and the substrate concentration in the aqueous/resin system，we found that a suitable aqueous/resin system with a matched load of resin can relieve substrate and product inhibition significantly and elevated the initial substrate concentration and product yield markedly. In an aqueous/resin system of 15 g/L resin AB-8，product yield could reach 58 % at the substrate concentration of 30 g/L with an 1.7 fold increase compared with the aqueous system.