Abstract: Three-dimensionally ordered macroporous (3DOM) carbon materials were successfully synthesized by using colloid crystal template which was self-assembled by sedimentation of SiO2 spheres with 540nm diameter. The voids between the SiO2 spheres were infiltrated with glucose solution as carbon precursor，and then the glucose was converted into carbon by carbonization. The template was removed with aqueous hydrofluoric acid. The obtained carbon materials exhibited a well-ordered array of hexagonally packed macropores with diameter of 510nm corresponding to a shrinkage of 5.6%. The macropores kept three-dimensionally interconnected macroporous networks through small windows. The walls of pores indicated a pore size distribution of mesopore with diameters of 2~10nm，which cooperated with the surface-templating replication resulted in a BET surface area of 948m2/g. The XRD spectrum showed the materials consisted of turbostratic graphite.