Abstract: Due to a series of food safety incidents，food safety has obtained more attention in our country. The detection of pathogenic microorganisms in food using immunomagnetic microsphere(IMMS) is more convenient and faster than conventional detection methods. The application principle and preparation methods of IMMS are reviewed. The application of IMMS for microorganism detection in food is further presented，and at the same time，the application of IMMS in conjunction with other detection methods such as PCR，ELISA，FIA and ECL applied in rapid pathogens detection is also introduced. This approach will become a trend in the development for food microorganism detection.