Abstract: Phenol-degradation bacteria were isolated from the soil of an agrochemical factory，their biodegradation ability for simulative phenolic wastewater was investigated in a 6 L bioreactor. The degradation of phenol with a concentration of 700 mg/L was up to 99% with a bacteria dosage of 7% and an aeration rate of 30 L/h at 17 ℃ and pH 6—8 within 24h. In the phnol concentration range between 200—700 mg/L，the average degradation rate increases with increasing the phenol concentration with a maximum of 29.2 mg/(L·h). The bacteria studied here are not sensitive to the variation in pH and the initial phenol concentration，and can adapt to a certain degree of impact load.