化工进展

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填充床反应器中酶法合成生物蜡酯

刘婷婷,邓 利,鲁吉珂,谭天伟,王 芳   

  1. 北京化工大学生命科学与技术学院,北京市生物加工过程重点实验室生物炼制教育部工程
    研究中心
  • 出版日期:2009-05-05 发布日期:2009-05-05

Synthesis of wax ester in an immobilized packed-bed reactor

LIU Tingting,DENG Li,LU Jike,TAN Tianwei,WANG Fang   

  1. College of Life Science and Technology,Beijing Key Laboratory of Bioprocess,Biorefinery Research and Engineering Center,Ministry of Education,Beijing University of Chemical Technology
  • Online:2009-05-05 Published:2009-05-05

摘要: 研究了填充床式反应器酶法合成生物蜡酯的生产工艺。以大豆油、十六醇为原料,在石油醚体系中,使用实验室自制的固定化Candida sp.99-125为催化剂。对操作参数,如填充高度、进料速度、固定化酶用量、底物摩尔比进行了研究。结果表明,固定化酶质量为47 g,填充高度40 cm(内径3.5 cm),油醇摩尔比1∶2.4,以4 mL/min速度进料保留时间4 h,产率可达83%。固定化酶在使用16批时,产率仍保持在70%以上。同时,对于填充床的填充方式作了初步探索。

Abstract: Wax ester was synthesized from soybean oil and cetyl alcohol in a packed-bed reactor with petroleum ether as solvent and immobilized lipase Candida sp.99-125 as catalyst. The effects of the height of packed-bed,flow rate of reactant,dosage of immobilized lipase,molar ratio of substrate were investigated. The optimum conditions were as follows:immobilized lipase 47g,the height of packed bed was 40cm (diameter 3.5cm),the molar ratio of oil to cetyl alcohol was 1∶2.4,the flow rate of reactant was 4mL/min and the retention time was 4h,the yield of wax ester reached 83% .The stability of the immobilized lipase was also studied,and the yield remained above 70% after 16 batches. The packed mode of the immobilized lipase in reactor was discussed.

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