Abstract: L-leucine can be deposited from hydrolysate of pig blood powder when sodium chloride (NaCl) is used as salting-out agent. In the deposition process, the time of salting-out deposition was determined, and influence of the main factors, such as temperature, pH value, and NaCl quantity on deposition efficiency was investigated. The results show that salting-out NaCl in the feed liquid. A low temperature was good for salting-out deposition, so industrial process can carried out at room temperature or low temperature. L-leucine is deposited as dileucine salt and the deposition efficiency reached a maximum when pH value of the solution is about 2 and NaCl was present. The greater the NaCl concentration in the solution, the higher the deposition efficiency. Addition of NaCl was 0.35g/mL in the industrial extraction process.