Abstract: A series of new carriers with different degrees of crosslinking were synthesized by suspension polymerization with glycidyl methacrylate (GMA) as functional monomer，ethylene glycol dimethacrylate (EGDMA) and divinyl benzene (DVB) as cross-linking agents. Mercury porosi-metry was used to characterize the pore structure（average pore diameter and surface area reached 14.1 nm，112.286 m2/g，respectively）. The swelling behavior of carriers in different solvents was also studied. At the same time，the functionalization of carrier was investigated，and the results showed that the carriers could react with amino group readily. The weak base exchange capacity，which reached 7.72 mmol/g in isopropanol was discussed. Finally，immobilization reaction of papain indicated that the activity of enzyme carriers was the highest in polyethylene polyamine，at a value of 2144 U/g.