Abstract: Whole cell immobilization can improve the stability of biocatalysts，simplify the separation process of oil and biocatalysts from the aqueous medium，and then lower the production costs of the biocatalysts. Therefore，the immobilization technique is of great importance for accelerating the industrialization process of deep biodesulfurization of petroleum feedstocks. The development of whole cell immobilization methods and reactors for biodesulfurization of petroleum feedstocks are reviewed. Current problems existed and corresponding suggestions are discussed.