Abstract: The adsorption behavior of bacteria on the surface of chalcopyrite was studied. The test results showed that the adsorption isotherms of T. f on chalcopyrite mineral surface could be described by the Freundlich equation. The culture age of the bacteria was in logarithmic growth anaphase with apparent attachment. pH value of 1.5～3.0，ionic strength of 50～100 mg/mL and non-ionic surfactant concentration of 0.005％～0.1％ were favorable to adsorption while the temperature had no evident effect on the attachment within the range of investigation. The adsorption kinetics was tested with the pseudo second order reaction model. The rate constant of adsorption for this kinetics model was calculated. Further analysis indicated that the pseudo second order chemical reaction model provided better correlation of data.