Abstract: Resin DA201 was screened out from several adsorbent resins. The resin had less adsorption ability for enzyme and coenzyme (adsorption rates 13.13% and 14.5% respectively) with no damge to enzyme activity，but at the same time，had strong adsorption ability for 1,3-PD and DHA (adsorption rates 23% and 27% respectively); The resin was used to adsorb and separate 1,3-PD and DHA，recycle enzyme and coenzyme to continue 1,3-PD enzyme catalyse with NADH regeneration; The conditon for the resin to have the preferable adsorption effect was mass of resin 4.8 times greater than 1g/L standard substrate，adsorption time 6 h. The 1,3-PD was continuously prepared by two batchs under this condition; 1,3-PD conversion rate was 42.9% and 35.5% for the first and second batches respectively，and total conversion rate was 39.72%; The result showed that the adsorption resin had certain separation effect for substrate，product，enzyme and coenzyme，and could effectively realize continuous enzyme catalyse reaction with coenzyme regeneration.