Abstract: 1,3-propenediol oxidoreductase isoenzyme is one of the key enzymes in biotransformation systhesis of 1,3-propenediol，and is encoded by the yqhD gene. YqhD gene was amplified from E. coli by polymerase chain reaction (PCR). The fragment was inserted into plasmid pET28 cut by two restriction enzymes BamHI and NheI. The yqhD gene after recombinant and transformation was induced and expressed by IPTG in E. coli at 25 ℃ for 12 h. The expression product was checked by SDS-PAGE. The cloned yqhD gene was obtained and protein expression was realized. The catalytic activity of 1,3-propenediol oxidoreductase isoenzyme was improved by 88 fold. The yield of 1,3-propenediol in whole cell fermentation with recombined strain could reach 65%，higher than wild strain (yield 9.5%).