Abstract: Bioconversion of L-phenylalanine to 2-phenylethylalcohol with yeasts was performed in an aqueous/organic solvent biphasic system. Saccharomyces cerevisiae CICIM Y0086（T）had more potential to transform L-phenylalanine to 2-phenylethylalcohol than Kluyvermyces marxianus AS.2.1440. The results of the solvent screening indicated that oleic acid was the best solvent which could extract 2-phenylethylalcohol efficiently among the four kinds of solvent. When the concentration of L-phenylalanine was 6 g/L，the optimum values of volume ratio of organic phase to aqueous phase，reaction temperature，pH value and liquid volume were 1∶1，30 ℃，4.0 and 20 mL/250 mL respectively. Under above optimum conditions，the content of 2-phenylethylalcohol reached 3.0 g/L 48 h later，which increased by about 50% than that in monophasic aqueous system.