化工进展

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两相体系生物转化L-苯丙氨酸生成2-苯乙醇

陆 军,张伟国   

  1. 江南大学工业生物技术教育部重点实验室
  • 出版日期:2008-03-05 发布日期:2008-03-05

Production of 2-phenylethylalcohol from L-phenylalanine by bioconversion in biphasic system

LU Jun,ZHANG Weiguo   

  1. Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University
  • Online:2008-03-05 Published:2008-03-05

摘要: 研究了水/有机溶剂两相体系中,酵母细胞转化L-苯丙氨酸(L-phe)生成2-苯乙醇(PEA)的过程。菌种选择表明酿酒酵母CICIM Y0086(T)的转化能力要高于马克斯克鲁维酵母AS.2.1440,有机溶剂的选择表明,在实验使用的4种有机溶剂中,油酸为该反应最适有机溶剂。当初始底物浓度为6 g/L、水和油酸的相体积比为1∶1、反应温度30 ℃和pH值4.0、装液量20 mL/250 mL 的条件下,转化48 h,产物浓度达到3.0 g/L,比单水相提高了50%。

Abstract: Bioconversion of L-phenylalanine to 2-phenylethylalcohol with yeasts was performed in an aqueous/organic solvent biphasic system. Saccharomyces cerevisiae CICIM Y0086(T)had more potential to transform L-phenylalanine to 2-phenylethylalcohol than Kluyvermyces marxianus AS.2.1440. The results of the solvent screening indicated that oleic acid was the best solvent which could extract 2-phenylethylalcohol efficiently among the four kinds of solvent. When the concentration of L-phenylalanine was 6 g/L,the optimum values of volume ratio of organic phase to aqueous phase,reaction temperature,pH value and liquid volume were 1∶1,30 ℃,4.0 and 20 mL/250 mL respectively. Under above optimum conditions,the content of 2-phenylethylalcohol reached 3.0 g/L 48 h later,which increased by about 50% than that in monophasic aqueous system.

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