Abstract: 1,3-propanediol (1,3-PD) is an important intermediate in the production of polyesters propylene terephthalate (PTT). Compared with the chemical method，the production by microbial fermentation has increasingly attracted more attention for its friendliness with the environment. In this paper，the research advances in genetic modification of Klebsiella pneumoniae are reviewed with regard to metabolic pathway，expression host，vector construction，and key enzymes in the conversion of glycerol to 1,3-propanediol. Moreover，some prevailing protocols for strain selection and genetic modification are also discussed.