Abstract: Biodiesel，a renewable，biodegradable，nontoxic and clean fuel，is made from plant oil or animal oil，and is expected to be a substitute for conventional diesel fuel. Biodiesel has the advantages of resolving energy shortage and reducing the emission of greenhouse gas. In this paper，the research progress of the activity and selectivity of lipase，immoblization of lipase，different reaction systems，operation conditions，the effect of different acyl acceptors and so on during enzymatic synthesis of biodiesel by lipase is presented.