Abstract: Isolated mixing regions (IMRs) exist widely in the stirred tank when operating with high- viscosity fluid under laminar flow conditions，which becomes a big barrier to efficient mixing. In order to increase the mixing efficiency，chaotic mixing was developed and applied successfully. The principle of chaotic mixing is dynamical perturbation，which can induce chaos within the flow field by destroying the periodicity of the moving fluid particles. Up to now，there are many methods about chaotic mixing in the stirred tanks，such as time-varying rotation，which includes co-reverse periodic rotation and time-periodically fluctuating rotation，eccentric rotation，reciprocating stirring，and so on. All the simulation and experimental research work have indicated that chaotic mixing can significantly improve the performance of the stirred tank and it has a great advantage over the conventional steady stirring methods.