Abstract: L-N-carbamoylase is one of the key biocatalysts in the enzymatic preparation of natural and non-natural amino acids with important potential and value in industry. The application of L-N-carbamoylase is limited due to the low thermal stability and activity. It is necessary to look for L-N-carbamoylase as a biocatalyst with high activity，high thermal stability and broad substrate specificity. In the paper，the application research，cloning and expression of L-N-carbamoylase，are reviewed.