Abstract: This paper presents the preparation methods of the biomimic membranes，which include the self-assembled approach，LB (Langmuir-Blodgett) technique，graft-modification technique，in-situ polymerization method，cast method，chemical vapor deposition method，and molecular deposition method. The research results of the cellular biomimic membranes，inorganic biomimic membranes，and hydrophobic biomimic membranes are given in this review; the practical application is also discussed. In the end，the latest trends in the study of biomimic membranes are predicted.