茄尼醇的SFC制备色谱分离工艺

化工进展

茄尼醇的SFC制备色谱分离工艺

耿中峰，吕惠生，张敏华

天津大学石油化工技术开发中心绿色合成与转化教育部重点实验室

出版日期:2007-10-25 发布日期:2007-10-25

Separation of solanesol by preparative-scale supercritical fluid chromatography

GENG Zhongfeng，LÜ Huisheng，ZHANG Minhua

Petrochemical Technology Development Center，Key Laboratory of Green Synthesis and Conversion，Ministry of Education，Tianjin University

Online:2007-10-25 Published:2007-10-25

摘要/Abstract

摘要： 采用ZORBAX SB-C18（250 mm×9.4 mm I.D.，5 μm）色谱柱为固定相，SC-CO2/甲醇为流动相，考察了超临界流体色谱分离提纯烟草萃取物中茄尼醇的工艺条件、流动相流速和改性剂含量对分离度的影响，以及温度和压力对茄尼醇容量因子、茄尼醇与相邻色谱峰组分分离度和选择性因子的影响。通过采用选定SFC分离工艺条件，茄尼醇产品纯度达到94.9%。

Abstract: Preparative-scale supercritical fluid chromatography was used to isolate solanesol from supercritical fluid extract. The chromatography conditions were as follows：ZORBAX SB-C18（250 mm×9.4 mm I.D.，5 μm）column was used as the steady phase; SC-CO2 with methanol as co-solvent was used as the mobile phase. The percent of modifier，temperature and pressure were investigated to get good resolution. In this paper，capacity factor and selectivity factor were also investigated. By chromatography separation，the concentration of solanesol reached 94.9%.

耿中峰，吕惠生，张敏华. 茄尼醇的SFC制备色谱分离工艺 [J]. 化工进展.

GENG Zhongfeng，LÜ Huisheng，ZHANG Minhua. Separation of solanesol by preparative-scale supercritical fluid chromatography[J]. Chemical Industry and Engineering Progree.