角质酶产生菌Thermobifida fusca WSH03-11 诱变及高产突变株发酵条件优化

化工进展

角质酶产生菌Thermobifida fusca WSH03-11
诱变及高产突变株发酵条件优化

张守亮，陈坚，华兆哲，堵国成

江南大学，工业生物技术教育部重点实验室；生物工程学院环境生物技术室

出版日期:2006-05-25 发布日期:2006-05-25

Mutagenesis of cutinase-producing strain Thermobifida fusca WSH03-11 and optimization of fermentation conditions of mutant with high cutinase production

ZHANG Shouliang，CHEN Jian，HUA Zhaozhe，DU Guocheng

Key Lab of Industrial Biotechnology，Ministry of Education； Environmental Biotechnology laboratory，
School of Biotechnology，Southern Yangtze University

Online:2006-05-25 Published:2006-05-25

摘要/Abstract

摘要： 以Thermobifida fusca WSH03-11为出发菌株，经硫酸二乙酯诱变，获得一株遗传稳定性好的高产角质酶的突变菌株(酶活由2.3 U/mL提高为8.9 U/mL)。确定了以乙酸钠为碳源，并在2.5 L发酵罐中研究了不同pH值对突变株角质酶分批发酵的影响。根据不同pH值下发酵过程中细胞比生长速率及产物比生长速率的变化，确定了分阶段控制pH值的策略，即在发酵前20 h控制pH值7.3、20 h后控制pH值7.6，这种条件下角质酶活达到19.8 U/mL，比采用单一pH值7.0、7.3、7.6、7.9下的最大值分别提高了125%，64%，37%，47%。

Abstract: Thermobifida fusca WSH03-11 was mutagenized by using DES and a mutant was isolated after three runs of screening. The cutinase activity was improved from 2.3 U/mL to 8.9 U/mL. After sodium acetate was selected as fermentation carbon source，batch microbial cutinase fermentation by a mutant of Thermobifida fusca WSH03-11 at various pH values ranging from 7.0 to 7.9 was studied. Based on time courses of the specific cell growth rate and specific cutinase formation rate at different pH values，a two-stage pH value-shift strategy in which pH value was controlled at 7.3 in the first 20 h，and then switched to 7.6 after 20 h was developed. By applying this pH value-shift strategy in cutinase fermentation，the maximal cutinase activity reached 19.8 U/mL and was increased by 125%，64%，37% and 47%，compared to those with constant pH values at 7.0，7.3，7.6 and 7.9，respectively.

张守亮，陈坚，华兆哲，堵国成. 角质酶产生菌Thermobifida fusca WSH03-11
诱变及高产突变株发酵条件优化
[J]. 化工进展.

ZHANG Shouliang，CHEN Jian，HUA Zhaozhe，DU Guocheng. Mutagenesis of cutinase-producing strain Thermobifida fusca WSH03-11 and optimization of fermentation conditions of mutant with high cutinase production[J]. Chemical Industry and Engineering Progree.