Abstract: The in vitro antitumor action of the different parts of ethanolic extractives from Camphor leaves，which were obtained by the technique of solvent-refining，was investigated in this paper. The dried Camphor leaves were submitted to ethanol（95%v/v）extraction and then the ethanolic extract was extracted again by petrol-ether，chloroform，ethyl acetate，methanol and water successively，and five extractives - A1，A2，A3，A4 and A5，were attained correspondingly. Using MTT and colony-forming methods，the five extractives were tested for the cytotoxicity on high metastatic lung carcinoma cells (95-D)，human oral epidermoid carcinoma (KB) and the human hepatoma cell line (HepG2). The extrac- A1，A2 and A3，showed the significant antitumor action when compared with the controls. The three extractives inhibited the tumor cells viability in concentration–dependent characteristics. These investigations suggested that the extract from camphor leaves had the function of antitumor in vitro，which was reported here firstly.