Abstract: The gold film electrodes were prepared by using two different methods. The surface modality of electrodes，and the self-assembling performance of single-strand DNA on the surface of electrodes were characterized with scanning electron microscope and Raman spectroscopy. The electrochemical characterization was made with cyclic voltammetry. The result showed that the performance of the electrodes prepared by using different methods was greatly different. When the elements diffused into each other on the surface of gold film electrodes，such phenomenon would adversely influence the electrochemical characterization of electrodes and the self-assembling performance of single-strand DNA on the surface of electrodes.