炼油废水中好氧反硝化菌的筛选及降解特性

doi:10.16085/j.issn.1000-6613.2016.05.039

化工进展 ›› 2016, Vol. 35 ›› Issue (05): 1524-1528.DOI: 10.16085/j.issn.1000-6613.2016.05.039

炼油废水中好氧反硝化菌的筛选及降解特性

武文丽, 颜家保, 陈佩, 霍晓琼, 吴优, 胡茜茜

武汉科技大学化学工程与技术学院, 湖北武汉 430081

收稿日期:2015-09-17 修回日期:2015-10-29 出版日期:2016-05-05 发布日期:2016-05-05
通讯作者: 颜家保,教授,博士生导师。E-mail 121135637@qq.com。
作者简介:武文丽(1990-),女,硕士研究生。
基金资助:
武汉科技大学研究生创新创业基金重点项目(JCX0006)及武汉科技大学国家级大学生创新创业训练计划项目(201310488009)。

Screening of an aerobic denitrifier from refinery wastewater and its characteristics

WU Wenli, YAN Jiabao, CHEN Pei, HUO Xiaoqiong, WU You, HU Qianqian

College of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, Hubei, China

Received:2015-09-17 Revised:2015-10-29 Online:2016-05-05 Published:2016-05-05

摘要/Abstract

摘要： 为了提高炼油废水生物处理装置的反硝化性能,从武汉石化污水处理厂的活性污泥中筛选出一株好氧反硝化菌AD10,经生理生化及16SrDNA序列分析,将其鉴定为假单胞菌属。通过单因素实验研究了该菌的最适培养条件:以丁二酸钠为碳源,C/N=14,温度30℃,初始pH=6.0,摇床转速200r/min。在此条件下,ρ(NO₃^--N)=556.81mg/L时72h的去除率为97.2%,且耐受的硝酸盐氮质量浓度达到654.00mg/L。菌株AD10培养11.5h,对ρ(NO₃^--N)=140.31mg/L和ρ(TN)=141.62mg/L的去除率分别为95.8%和93.9%,其反硝化速率高于大部分已发现的好氧反硝化菌,检测到的ρ(NO₂^–-N)≤2.30mg/L,未出现明显的积累,菌株AD10能够进行完全反硝化。

关键词: 炼油废水, 假单胞杆菌, 好氧反硝化, 硝酸盐氮, 总氮

Abstract: In order to improve the denitrification efficiency of the bioreactor of refinery wastewater treatment plant, an aerobic denitrifier AD10 was isolated from the activated sludge of a petrochemical wastewater treatment plant in wuhan city. It was identified as a member of the genus Pseudomonas sp. according to its morphyological and physiological properties and 16S rDNA gene sequence analysis. Through the single factor experiment investigations, the optimum denitrification conditions were: sodium succinate as carbon source, C/N 14, temperature 30℃, initial pH 6.0, rotation speed 200 r/min. Under these conditions, the nitrate remove efficiency of AD10 was 97.2% when it was cultivated in 556.81 mg/L nitrate for 72h, and it could tolerate at least 654 mg/L of nitrate nitrogen. Culturing after 11.5h, AD10 show the NO₃^–-N and TN removal rate of 95.8% and 93.9%, when the concentration of NO₃^--N and TN were 140.31mg/L and 141.62mg/L, respectively. AD10's denitrification rate is higher than most of the aerobic denitrifying bacteria have been found. During this process, only a very small amount of NO₂^–-N (≤2.30mg/L) was detected, indicating a complete denitrification.

Key words: refinery wastewater, Pseudomonas sp., aerobic denitrification, nitrate nitrogen, total nitrogen

中图分类号:

X172

武文丽, 颜家保, 陈佩, 霍晓琼, 吴优, 胡茜茜. 炼油废水中好氧反硝化菌的筛选及降解特性[J]. 化工进展, 2016, 35(05): 1524-1528.

WU Wenli, YAN Jiabao, CHEN Pei, HUO Xiaoqiong, WU You, HU Qianqian. Screening of an aerobic denitrifier from refinery wastewater and its characteristics[J]. Chemical Industry and Engineering Progree, 2016, 35(05): 1524-1528.

参考文献

[1] 刘欢, 骆灵喜, 李旭宁, 等.膜生物反应器好氧反硝化菌的筛选及鉴定[J].环境污染与防治, 2015, 37(4): 26-29.
[2] ROBERTSON L A, VANNEIL E W J, TORREMANS R A M, et al. Simultaneous nitrification and denitrification in aerobic chemostat cultures of Thiosphaera pantotroph[J]. Appl. Environ. Mcrobiol., 1983, 54(11): 2812-2818.
[3] 王弘宇, 马放, 苏俊峰, 等.好氧反硝化菌株的鉴定及其反硝化特性研究[J].环境科学, 2007, 28(7): 1548-1552.
[4] 李兵, 林炜铁.1株好氧反硝化芽孢杆菌的脱氮特性研究[J].水生态学杂志, 2009, 2(3): 48-51.
[5] 杨冠东, 钟瑜, 石笛, 等.制革废水中好氧反硝化菌的筛选及其特性[J].化工环保, 2013, 33(5): 402-405.
[6] 张亚光, 方柏山, 闵航, 等.一株好氧反硝化菌的特征及系统进化分析[J].华侨大学学报, 2004, 25(1): 75-78.
[7] 乔楠, 陈瑞佳, 于大禹.硅藻土负载异养硝化-好氧反硝化菌的脱氮性能[J].化工进展, 2015, 34(5): 1459-1465.
[8] TUO Baohua, YAN Jiabao, FAN Baoan, et al. Biodegradation characteristics and bioaugmentation potential of a novel quinoline-degrading strain of Bacillus sp. isolated from petroleum-contaminated soil[J]. Bioresour Technol., 2012, 107: 55-60.
[9] 东秀珠, 蔡妙英.常见细菌系统鉴定手册[M].北京: 科学出版社, 2001: 180-181.
[10] 原国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].北京: 中国环境科学出版社, 2000: 279-281.
[11] 林海.环境工程微生物学[M].北京: 冶金工业出版社, 2008: 73-74.
[12] 李慧颖, 黄少斌, 范利荣.一株好氧反硝化菌的反硝化性能研究[J].环境科学与技术, 2009, 32(8): 9-12.
[13] 周云云, 黄少斌.好氧反硝化菌TADl的同步硝化反硝化性能[J].环境工程学报, 2013, 7(6): 2160-2166.
[14] 王薇, 蔡祖聪, 钟文辉, 等.好氧反硝化菌的研究进展[J].应用生态学报, 2007, 18(11): 2618-2625.
[15] 周影茹, 陆玉芳, 施卫明.一株根际好氧反硝化菌的筛选及其反硝化条件研究[J].土壤, 2013, 45(4): 683-690.
[16] 王慧荣, 韦彦斐, 梅荣武, 等.一株好氧反硝化菌的分离及特性研究[J].环境保护科学, 2012, 38(4): 13-18.
[17] 高喜燕, 刘鹰, 郑海燕, 等.一株海洋好氧反硝化菌的鉴定及好氧反硝化特性[J].微生物学报, 2010, 50(9): 1164-1171.

炼油废水中好氧反硝化菌的筛选及降解特性

Screening of an aerobic denitrifier from refinery wastewater and its characteristics

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

本文评价

[1]	胡杰,颜家保,霍晓琼,陈美玲,李超. 低C/N比异养硝化-好氧反硝化菌筛选及硝化特性[J]. 化工进展, 2019, 38(03): 1567-1572.
[2]	赵慧敏, 赵剑强. 不同运行方式对微生物燃料电池处理氨氮废水的影响[J]. 化工进展, 2016, 35(05): 1549-1554.
[3]	乔楠, 陈瑞佳, 于大禹. 硅藻土负载异养硝化-好氧反硝化菌的脱氮性能[J]. 化工进展, 2015, 34(05): 1459-1465.
[4]	于大禹1，张琳颖1，高　波1，2. 异养硝化-好氧反硝化菌异养硝化性能的影响因素[J]. 化工进展, 2012, 31(12): 2797-2800.
[5]	李芳1，2，崔红梅1，2,吕炳南3. 生物转盘同步去除化学需氧量和氮的实验研究[J]. 化工进展, 2012, 31(07): 1615-1619.
[6]	乔楠1，刘文超2，张贺2，张金榜2，于大禹2. 一株好氧反硝化菌的异养硝化及脱氮性能 [J]. 化工进展, 2010, 29(4): 767-.
[7]	乔楠，张静，张金榜，孙伟，于大禹. 纳米Fe₃O₄负载好氧反硝化菌脱氮除磷性能 [J]. 化工进展, 2009, 28(11): 2058-.