Chemical Industry and Engineering Progress ›› 2021, Vol. 40 ›› Issue (5): 2710-2718.DOI: 10.16085/j.issn.1000-6613.2020-1289

• Biochemical and pharmaceutical engineering • Previous Articles     Next Articles

Controlled/“living” radical polymerization-based biosensing

HU Qiong(), GAN Shiyu, BAO Yu, HAN Dongxue, NIU Li()   

  1. Center for Advanced Analytical Science, School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, Guangdong, China
  • Received:2020-07-08 Online:2021-05-24 Published:2021-05-06
  • Contact: NIU Li

基于可控/“活性”自由基聚合的生物传感分析

胡琼(), 甘世宇, 包宇, 韩冬雪, 牛利()   

  1. 广州大学化学化工学院,分析科学技术研究中心,广东 广州 510006
  • 通讯作者: 牛利
  • 作者简介:胡琼(1988—),男,博士,副教授,研究方向为电化学生物传感。E-mail:q.hu@gzhu.edu.cn
  • 基金资助:
    国家自然科学基金青年科学基金(21904026);中国博士后科学基金第64批面上项目(2018M643040)

Abstract:

The defects of high cost and complex operation of conventional signal amplification strategies are adverse to their potential applications in early diagnosis and other fields. In recent years, a new kind of signal amplification strategies based on the use of controlled/“living” radical polymerization (CLRP) techniques such as atom transfer radical polymerization (ATRP) and reversible addition-fragmentation chain transfer (RAFT) polymerization has been established for the simple, fast, low-cost, highly sensitive and selective detection of biological molecules (e.g., proteins and nucleic acids). In this paper, the research progress of the CLRP-based biosensing was reviewed. Firstly, the concept and characteristics of biosensors were introduced, as well as the pros and cons of the conventional and the polymer-based amplification strategies. Furthermore, the application of the ATRP-based and the RAFT polymerization-based amplification strategies in the highly sensitive biosensing was reviewed. Finally, the prospect of the CLRP-based biosensing was presented. By virtue of their advantages such as simple operation, low cost, and high efficiency, the CLRP-based amplification strategies show great potential in the highly sensitive detection of biological molecules.

Key words: electrochemistry, biosensor, polymerization, radical, signal amplification, atom transfer radical polymerization, reversible addition-fragmentation chain transfer (RAFT) polymerization

摘要:

传统信号放大策略存在成本高昂和操作复杂等不足,无法满足早期诊断等领域的实际应用需求。近年来,科研人员探索建立了一类基于原子转移自由基聚合(ATRP)和可逆加成-断裂链转移(RAFT)聚合等可控/“活性”自由基聚合(CLRP)技术的新型信号放大策略,用于蛋白质和核酸等生物分子的简便、快速、低成本、高灵敏和高选择性检测。本文综述了基于CLRP的生物传感分析的研究进展。首先介绍了生物传感器的概念及特点,简述了传统信号放大策略及基于聚合物的信号放大策略的优缺点。接着,对CLRP技术进行了概述,并重点回顾了基于ATRP和RAFT聚合的信号放大策略在高灵敏生物传感中的应用。最后,对基于CLRP的生物传感分析进行了展望。基于CLRP的信号放大策略,具有操作简便、成本低廉和高效等优良特性,在生物分子的高灵敏检测中具有相当广阔的应用前景。

关键词: 电化学, 生物传感器, 聚合, 自由基, 信号放大, 原子转移自由基聚合, 可逆加成-断裂链转移聚合

CLC Number: 

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