Comparative study on the antibacterial properties of TiO2, ZnO and TiO2/ZnO oxide powders

doi:10.16085/j.issn.1000-6613.2017-2607

Abstract

Abstract: Due to their high chemical stability and rich and easy raw material availability, oxides as antibacterial materials are receiving increasing attention. In-depth study of the antibacterial properties of oxides and the related influence factors can provide a scientific basis to explore new antibacterial materials. TiO₂/ZnO composite was prepared by sol-gel method, and was compared with TiO₂ and ZnO. The crystal structure, morphology and particle size of these materials were characterized. Using the Escherichia coli ATCC25922 as the tested strain, we investigated the antibacterial activities of these three oxides through bacterial inhibition zone test and bacterial suspension contact test. The effects of titanium-zinc ratio, light and other conditions on the antibacterial properties were also investigated. It was found that the TiO₂ sample was mainly composed of anatase phase and rutile phase, while the ZnO sample was mainly of hexagonal wurtzite phase. And it is mainly the anatase TiO₂ depositing in the pore of hexagonal wurtzite ZnO in TiO₂/ZnO composite. The powder size of the three samples were in a descending order of TiO₂/ZnO, TiO₂ and ZnO. Both bacterial inhibition zone test and bacterial suspension contact test confirmed that the antibacterial performance of the ZnO sample was obviously better than that of the TiO₂. The antibacterial performance of ZnO and TiO₂/ZnO were both good under dark conditions, but became even better under visible light. The comparative analysis demonstrated that the content of ZnO in the TiO₂/ZnO composite played a major role in the antibacterial effect. The calcination temperature during the preparation of TiO₂/ZnO did not show significant effect on its antibacterial performance.

Key words: titanium dioxide (TiO₂), zinc oxide (ZnO), TiO₂/ZnO, antibacterial, powders, composites, preparation

摘要： 氧化物具有化学性质稳定、原材料丰富易得的优点，其作为抗菌材料日益受到关注。对氧化物的抗菌性能及影响因素开展深入研究，能够为新型抗菌材料的开发提供科学依据。通过溶胶-凝胶法制备出了TiO₂/ZnO复合粉体，并将其与TiO₂、ZnO进行对比，分别对它们的晶相结构、微观形貌和粒径进行了表征。利用大肠杆菌ATCC25922作为受试菌株，通过抑菌环试验和菌液接触试验对3种氧化物粉体材料的抗菌性能进行测试。考察了钛锌比、光照等条件对抗菌效果的影响。结果表明，所使用的TiO₂主要由锐钛矿相和金红石相组成，ZnO主要为六方纤锌矿相，制备出的TiO₂/ZnO则主要是锐钛矿型TiO₂在六方纤锌矿型ZnO的孔隙间沉积而成，这3种粉体材料的粒径由大到小分别为TiO₂/ZnO、TiO₂和ZnO。从抑菌环和菌液接触试验的结果可以看出，ZnO粉体材料的抗菌效果明显优于TiO₂粉体材料，ZnO和TiO₂/ZnO在黑暗条件下仍有良好的抗菌性能，但在可见光照射条件下抗菌效果更佳。ZnO含量是TiO₂/ZnO复合材料抗菌性能的关键因素。制备TiO₂/ZnO粉体材料时的煅烧温度对其抗菌性能没有明显影响。

关键词: 二氧化钛, 氧化锌, 二氧化钛/氧化锌, 抗菌, 粉体, 复合材料, 制备

CLC Number:

TB34

ZHANG Chongmiao, WEN Yinmei, GAO Min, GAO Qian. Comparative study on the antibacterial properties of TiO₂, ZnO and TiO₂/ZnO oxide powders[J]. Chemical Industry and Engineering Progress, 2018, 37(11): 4343-4348.

张崇淼, 温银梅, 高敏, 高倩. TiO₂、ZnO和TiO₂/ZnO三种氧化物粉体材料的抗菌性能对比[J]. 化工进展, 2018, 37(11): 4343-4348.

References

[1] 莫尊理, 胡惹惹, 王雅雯, 等. 抗菌材料及其抗菌机理[J]. 材料导报A:综述篇, 2014, 1(28):50-52. MO Zunli, HU Rere, WANG Yawen, et al. Review of antibacterial materials and their mechanisms[J]. Material Review A, 2014, 1(28):50-52.
[2] GAO Likun, GAN Wentao, XIAO Shaoliang, et al. A robust superhydrophobic antibacterial Ag-TiO₂ composite film immobilized on wood substrate for photodegradation of phenol under visible-light illumination A robust superhydrophobic antibacterial Ag-TiO₂ composite film immobilized on wood substrate for photodegradation of phenol under visible-light illumination[J]. Ceramics International, 2016, 42:2170-2179.
[3] 张凤君, 刘卓婧, 刘兆煐, 等. TiO₂光催化剂改性研究进展[J]. 科技导报, 2013, 31(17):66-71. ZHANG Fengjun, LIU Zhuojing, LIU Zhaohuan, et al. Review on the modification of TiO₂ photocatalyst[J]. Science and Technology Review, 2013, 31(17):66-71.
[4] CHATTERJEE Debabrata, MAHATA Anima. Photoassisted detoxification of organic pollutants on the surface modified TiO₂ semiconductor particulate system[J]. Catalysis Communications, 2001, 2(1):1-3.
[5] CHOI W, TERMIN A, HOFFMANN R M. The role of metal ion dopants in quantum -sized TiO₂:correlation between photoreactivity and charge carrier recombination dynamics[J]. Journal of Chemical Physics, 1994, 98(51):13669-13679.
[6] CHENG Ping, LI Wei, ZHOU Tianle, et al. Physical and photocatalytic properties of zinc ferrite doped titania under visible light irradiation[J]. Journal of Photochemistry and Photobiology A:Chemistry, 2004, 168(1/2):97-101.
[7] SIRELKHATIM Amna, SHAHROM Mahmud, AZMAN Seeni. Review on zinc oxide nanoparticles:antibacterial activity and toxicity mechanism[J]. Nano-Micro Lett., 2015, 7(3):219-242.
[8] XIE Y P, HE Y P, PETER L I, et al. Antibacterial activity and mechanism of action of zinc oxide nanoparticles against Campylobacter jejuni[J]. Applied and Enviornmental Microbiology, 2011, 77(7):2325-2331.
[9] ZHANG Lingling, LI Yu, LIU Xiaoming, et al. The properties of ZnO nanofluids and the role of H₂O₂ in the disinfection activity against Escherichia coli[J]. Water Research, 2013, 47:4013-4021.
[10] ŠTRBAC D, AGGELOPOULOS C A, ŠTRBAC G, et al. Photocatalytic degradation of naproxen and methylene blue:comparison between ZnO, TiO₂ and their mixture[J]. Process Safety and Environment Protection, 2018, 113:174-183.
[11] 贾振斌, 陈伙德, 邱敏. 纳米ZnO-TiO₂复合粉体的制备及抗菌性能的研究[J]. 广东化工, 2012, 9(39):83-84. JIA Zhenbin, CHEN Huode, QIU Min. Study on preparation and antibacterial activity of nano-ZnO/TiO₂ composite powders[J]. Guangdong Chemical Industry, 2012, 9(39):83-84.
[12] 胡亚微, 贺惠蓉, 马养民, 等. TiO₂/ZnO粒子的制备及其抗菌性能[J]. 化工新型材料, 2014, 42(7):77-79. HU Yawei, HE Huirong, MA Yangmin, et al. Fabrication and antibacterial activity of TiO₂/ZnO paticles[J]. New Chemical Materials, 2014, 42(7):77-79.
[13] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会.纳米无机材料抗菌性能检测方法:GB/T 21510-2008[S].北京:中国标准出版社, 2008. General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China. Antimicrobial property detection methods for nano-inorganic materials:GB/T 21510-2008[S]. Beijing:Standards Press of China, 2008.
[14] QI Kezhen, CHENG Bei, YU Jiaguo, et al. Review on the improvement of the photocatalytic and antibacterial activities of ZnO[J]. Journal of Alloys and Compounds, 2017, 727:792-820.
[15] SOURABH Dwivedi, RIZWAN Wahab, FARHEEN Khan, et al. Reactive oxygen species mediated bacterial biofilm inhibition via zinc oxide nanoparticles and their statistical determination[J]. PLOS ONE, 2014, 9(11):111-289.
[16] JONES Nicole, RAY Binata, RANJIT Koodali T, et al. Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms[J]. FEMS Microbiol. Lett., 2008, 279(1):71-76.
[17] HIROTA Ken, SUGIMOTO Maiko, KATO Masaki, et al. Preparation of zinc oxide ceramics with a sustainable antibacterial activity under dark conditions[J]. Ceram. Int., 2010, 36(2):497-506.
[18] HEINAAN Margit, IVASK Angela, BLINOVA Irina, et al. Toxicity of nanosized and bulk ZnO, CuO and TiO₂ to bacteria Vibrio fischeri and crustaceans Daphnia magna and Thamnocephalus platyurus[J]. Chemosphere, 2008, 71(7):1308-1316.
[19] XIA Tian, MICHAEL Kovochich, MONTY Liong. Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties[J]. ACS Nano., 2008, 2(10):2121-2134.
[20] KAJA K, ANGELA I, HENRI-CHARLES D, et al. Toxicity of nanoparticles of ZnO, CuO and TiO₂ to yeast Saccharomyces cerevisiae[J]. Toxicology in Vitro, 2009, 23:1116-1122.
[21] YANG Hui, LIU Chao, YANG Danfeng, et al. Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by fourtypical nanomaterials:the role of particle size, shape and composition[J]. J. Appl. Toxicol., 2009, 29:69-78.
[22] WAGHMARE M A, PAWAR K S, PATHAN H M. Influence of annealing temperature on the structural and optical properties of nanocrystalline zirconium oxide[J]. Materials Science in Semiconductor Processing, 2017, 72:122-127.
[23] PINJARI D V, PRASAD K, GOGATE C, et al. Synthesis of titanium dioxide by ultrasound assisted sol-gel technique:effect of calcination and sonication time[J]. Ultrasonics Sonochemistry, 2015, 23:185-191.
[24] ASHRAF Robina, RIAZ Saira, KAYANI Z N, et al. Effect of calcination on properties of ZnO nanoparticles[J]. Materials Today:Proceedings, 2015, 2:5468-5472.

Comparative study on the antibacterial properties of TiO₂, ZnO and TiO₂/ZnO oxide powders

TiO₂、ZnO和TiO₂/ZnO三种氧化物粉体材料的抗菌性能对比

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