Abstract: Carbon dioxide is the main greenhouse gas，and rapid development has been made in the study of fixation and conversion of CO2 in recent years. Chemical and biological methods are the main methods of CO2 fixation. The low efficiency，high energy consumption，and high requirements of equipment performance are the significant disadvantages of chemical conversion of CO2. Biological methods of CO2 fixation include enzymatic and microalgal carbon dioxide fixation，which have attracted much attention due to their mild reaction conditions and pollution-free. This paper summarizes the research status of CO2 fixation using multi-enzyme system and microalgae at home and abroad，focusing on the research progress of choosing and optimizing of multi-enzyme system，screening and breeding of genetic engineered microalgae，and the design of photoreactor and the synthesis of high value-added products. Meanwhile，CO2 fixation coupled with production of useful substances by building new multi-enzyme system is the primary development direction of enzymatic carbon dioxide fixation. In addition，the construction of genetic engineering micro-algae and the development of new photoreactor are efficient methods to improve microalgal carbon sequestration.

Key words: carbon dioxide, enzyme, microalgae

摘要： CO2是导致温室效应的主要气体，固定和转化CO2的研究近年来取得了迅猛发展。目前，CO2转化的主要方法为化学法和生物法，化学法转化CO2存在转化效率低、能耗高、对设备耐温耐压性能要求高等缺点，生物法主要是指酶法及微藻固定CO2，其由于反应条件温和且绿色无污染的优点而备受关注。本文综述了国内外在多酶体系和微藻固定CO2方面的研究现状，重点对多酶体系的选择和优化、微藻筛选与基因工程育种、光反应器设计及高附加值产品的合成等方面的研究情况进行了论述，并指出构建新的多酶体系将CO2固定与有用物质生产相偶联是生物酶法的主要发展方向，此外，基因工程微藻的构建及新型光反应器的研发是提高微藻法固碳效率的主要方式。

关键词: 二氧化碳, 酶, 微藻