Abstract: Taking anaerobic bacteria from domestic waste water as biocatalyst，glucose as fuel，a double-chamber microbial fuel cell (MFC) was constructed. The battery was operated for 5 cycles intermittently.The effect of temperature on its producing electricity characteristics was investigated by electrochemical test methods，including cyclic voltammogram，alternating current impedance and polarization test. In a specific temperature range，electrochemical activity of the microbe improved with increasing temperature，power density and exchange current density were enhanced，while charge transfer impedance decreased. At 32 ℃ the battery showed the best performance. Power density and exchange current density reached 156.2 mW/m2 and 8.02×10?5 mA/m2 at 32 ℃，respectively. However，it was not beneficial to bacterial activity at a too low or too high temperature. When the temperature of the battery was at 18 ℃，25 ℃，32 ℃，39 ℃ and 46 ℃，the percentages of load transfer resistance in anode resistance were 97.99%，84.02%，47.36%，91.30% and 99.61%，respectively. It demonstrated that charge transfer impedance occupied the overwhelming share of total resistance at the anode. MFC was an electrochemical system under the control of charge transfer process.

Key words: microbial fuel cell (MFC), fuel cell, wastewater, temperature

摘要： 实验采用双室型微生物燃料电池（MFC），以生活废水中厌氧菌作为生物催化剂，葡萄糖为燃料，通过5个不同温度条件下的间歇运行，应用循环伏安、交流阻抗、极化测试等电化学方法考察温度对电池产电性能的影响。结果表明，一定温度范围内，提高温度有助于增强微生物的电化学活性，降低传荷阻抗，提高电池输出功率密度和交换电流密度。32 ℃时，电池产电效能最佳，电池功率密度和交换电流密度分别达到156.2 mW/m2和8.02×10?5 mA/m2，温度太低或太高均不利于细菌的电化学活性。体系温度为18 ℃、25 ℃、32 ℃、39 ℃、46 ℃时，传荷阻抗Rct在阳极内阻中占的比例分别为97.99%、84.02%、47.36%、91.30%、99.61%，说明传荷阻抗在阳极内阻中占绝对份额，MFC是传荷过程控制下的电化学反应体系。

关键词: 微生物燃料电池, 燃料电池, 废水, 温度