Abstract: To optimize the formulation parameters of beta-carotene loaded MCM-41 mesoporous silica for the maximum encapsulation efficiency,experiments were designed according to a three-level,three-variable Box-Behnken design (BBD). Independent variables were the amount of pore enlarging agent (A),the beta-carotene/MCM-41 mass ratio (B),and loading time (C),and the response variable was the encapsulated efficiency of beta-carotene (Y_EE). The optimal formulation parameters were as follows:A,B and C levels were 0.6mL,3 and 20h,respectively. The observed responses agree with the predicted values of the mathematic models,and the Box-Behnken design is suitable for optimizing the formulation of beta-carotene loaded MCM-41 mesoporous silica.

Key words: MCM-41, β-carotene, Box-Behnken design

摘要： 采用三水平三因素的Box-Behnken实验设计,利用响应曲面法对影响MCM-41包载β-胡萝卜素包封率的主要因素进行研究,主要考虑的影响因素有载药时间、扩孔剂含量、β-胡萝卜素/MCM-41投药比等,以MCM-41对β-胡萝卜素的包封率作为评价因素,建立相应的数学模型,优化处方。结果表明,最优的处方工艺是载药时间为20h、扩孔剂含量为0.6mL、β-胡萝卜素/MCM-41投药比为3。在此条件下,β-胡萝卜素的包封率可达理论预测值的95.83%,说明将Box-Behnken实验设计法用于MCM-41包载β-胡萝卜素处方的优化筛选是可行的,且得到的实验观察值与数学模型的预测值相符合。

关键词: MCM-41, &beta, -胡萝卜素, Box-Behnken实验设计