Abstract: Mannitol，which is the main component of brown algae，was utilized in this study to produce butanol by Clostridium acetobutylicum (ATCC 824). Plackett-Burman (P-B) and Box-Behnken designs were adopted to optimize the fermentation conditions. Ten factors，including beef extract，yeast extract，tryptone，ammonium acetate，KH2PO4，MgSO4?7H2O，FeSO4?7H2O，size of inoculum，fermentation temperature and initial pH，were considered to affect butanol production in the Plackett-Burman (P-B) design. The result demonstrated that fermentation temperature，initial pH and concentration of ammonium acetate were the most critical factors，and optimal fermentation conditions were 37.3℃，pH 6.38 and 2.82 g/L of ammonium acetate according to the Box-Behnken design. A mathematical model was established and used to predict the maximum butanol yield of 8.47 g/L. The result of verification experiment showed that the maximum butanol yield reached 8.52±0.55g/L under the optimum conditions，which showed that the statistical method was a useful tool for butanol production from mannitol in the optimization.

Key words: mannitol, butanol , Plackett-Burman, Clostridium acetobutylicum , Box-Behnken, mannitol, butanol, Plackett-Burman design, Box-Behnken design, Clostridium acetobutylicum

摘要： 以褐藻的主要成分甘露醇为底物，利用丙酮丁醇梭菌ATCC824发酵生产丁醇。采用Plackett-Burman（P-B）和Box-Behnken优化发酵条件。在Plackett-Burman实验设计时，考察牛肉膏、酵母浸粉、胰蛋白胨、乙酸铵、 KH2PO4、MgSO4?7H2O、FeSO4?7H2O、接种量、发酵温度、初始pH值这10个因素对丁醇产量影响，筛选出影响丁醇发酵的重要参数是发酵温度、初始pH值、乙酸铵浓度，利用Box-Behnken设计确定最优发酵条件为发酵温度37.3 ℃、初始pH值6.38、乙酸铵浓度2.82 g/L。数学模型分析预测最大丁醇产量为8.47 g/L。经实验验证表明，在优化条件下的丁醇产量平均值达到8.52±0.55 g/L。这说明利用统计学方法优化甘露醇的丁醇发酵条件是有效的。

关键词: 甘露醇, 丁醇, Plackett-Burman, Box-Behnken, 丙酮丁醇梭菌, 甘露醇, 丁醇, Plackett-Burman, Box-Behnken, 丙酮丁醇梭菌