Abstract: In recent years，a method to produce shape-specific nanoparticles which can load any cargo has become a hot and difficult area of research. This review starts with a brief introduction of the advanced nanoparticles，which can load any cargo. A novel method called PRINT (particle replication in non-wetting templates) can be used to design and prepare the templates through the low surface energy perfluoropolyether network. By the confined space of template，shape-controlled particles are fabricated. The method of nanofabrication and application in loading anti-cancer drug is summarized. The anti-cancer drug particles with different haspect ratios contributes to different drug release rates. This breakthrough in nanotechnology will have tremendous potential for applications in nanomedicine and diagnostics.

Key words: templates, nanoparticles, drug delivery, non-wetting templates, nanoparticles, drug delivery

摘要： 近年来，能够精确控制尺寸、形态、组成的纳米微粒制备方法的研究成为科学研究的难点与热点。本文简述了一项新型的纳米微粒制备技术：非浸润模板微印制技术（PRINT，particle replication in non-wetting templates），该技术是将微细加工技术应用到材料合成领域，以不亲水不亲油的含氟材料为模板，预先根据所需微结构的尺寸、形态设计制作模板，利用模板的空间局限作用，压印制备特定尺寸、形态、取向、排布的单分散载药微粒。重点阐述了PRINT技术微粒制备的方法以及在抗癌载药微粒的制备与应用，最后总结指出由PRINT技术制备的多种不同高宽比的形态抗癌载药微粒在病体细胞中具有不同的释药效果，该项技术在纳米微粒制备领域的突破有望推动纳米医药与生物诊断研究领域的巨大发展。

关键词: 非浸润工艺, 纳米微粒, 载药微粒, 非浸润工艺, 纳米微粒, 载药微粒